Pro BDNF antibody (Pro-Domain)

Catalog No. ABIN7043557

This Guinea Pig Polyclonal antibody specifically detects Pro BDNF in IHC, IF and WB. It exhibits reactivity toward Human.

Quick Overview for Pro BDNF antibody (Pro-Domain) (ABIN7043557)

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Reactivity: Human

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Pro BDNF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB)

Product Details anti-Pro BDNF Antibody

Binding Specificity: AA 72-88, Pro-Domain

Purpose: A Guinea Pig Polyclonal Antibody to proBDNF

Cross-Reactivity: Human, Mouse

Cross-Reactivity (Details): The antibody is specific for proBDNF, it does not crossreact with proNGF, proNT-3 or mature BDNF.

Predicted Reactivity: Mouse,rat - 16,17 amino acid residues identical

Characteristics: Guinea pig Anti-proBDNF Antibody is directed against the prodomain region of human proBDNF. Guinea pig Anti-proBDNF Antibody , raised in guinea pig, can be used in western and immunohistochemistry applications. In addition, the antibody is an excellent tool for easy multiplex staining studies. It has been designed to recognize proBDNF from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-proBDNF Antibody (ABIN7043558, ABIN7044753 and ABIN7044754) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor)

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Comment:

Positive Control: PCP-B257

Negative Control: (ABIN7236605)

Blocking Peptide: (ABIN7236605)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Pro BDNF

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Alternative Name: proBDNF

Background:

Synonyms: Brain-derived neurotrophic factor precursor

Description: Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the prodomain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP), a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimer's disease.Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. proBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.

Gene ID: 627

NCBI Accession: NM_170735

UniProt: P23560