PRLHR antibody (AA 247-260)

Catalog No. ABIN7043562

This anti-PRLHR antibody is a Rabbit Polyclonal antibody detecting PRLHR in WB. Suitable for Rat.

Quick Overview for PRLHR antibody (AA 247-260) (ABIN7043562)

Target: PRLHR (Prolactin Releasing Hormone Receptor (PRLHR))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRLHR antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRLHR Antibody

Binding Specificity: AA 247-260

Purpose: A Rabbit Polyclonal Antibody to Prolactin-Releasing Peptide Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-PrRP Receptor (GPR10) Antibody is directed against an epitope of the rat Prolactin-releasing peptide receptor. Anti-PrRP Receptor (GPR10) Antibody (ABIN7043562, ABIN7045116 and ABIN7045117) can be used in western blot analysis. It has been designed to recognize PrRPR from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RVSVKLRNRVVPGS, corresponding to amino acid residues 247-260 of rat PRLHR

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:1000

Comment:

Negative Control: (ABIN7236617)

Blocking Peptide: (ABIN7236617)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PRLHR

Target: PRLHR (Prolactin Releasing Hormone Receptor (PRLHR))

Alternative Name: PRLHR

Background: Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR,Prolactin-releasing peptide (PrRP) a bioactive peptide, was originally identified as an endogenous ligand of the orphan human G-protein coupled receptor, seven transmembrane domain receptor, GPR10 (hGR3, also called PrRP receptor). Like the majority of orphan receptors, PrRP receptor shows a low level of sequence homology with known GPCRs. Notwithstanding, a certain degree of homology has been reported to members of the neuropeptide Y (NPY) family of receptors. Neuropeptide Y is a 36- residue C-terminally amidated polypeptide hormone and neurotransmitter with the presence of an α-helix involving the 20 C-terminal residues, whereas the N-terminus part seems more flexible and is influenced by the medium environment1. It has been recently demonstrated that the PrRP receptor may modulate neurotransmission specifically at glutamatergic synapses through the interaction with the same PDZ domain proteins that interact with AMPA receptors2.Binding of PrRP to the receptor results in activation of extracellular signal-related kinase (ERK) in a mainly pertussis toxin sensitive manner, suggesting coupling to Gi/o proteins.PrRP receptor is expressed in the central nervous system with highest levels in the pituitary. Expression has also been detected in the cerebellum, brainstem, hypothalamus, thalamus, nucleus tractus solitaries, adrenal medulla and spinal cord in rat3.PrRP is implicated in the regulation of the hypothalamic-pituitary-adrenal axis, blood pressure and nociception4,5. Genetic studies in rodents indicate that lack of PrRP receptor leads to hyperphagia, obesity and dyslipidemia4. In humans, genetic variations in PrRP receptor are associated with lowered blood pressure5.

Alternative names: PrRP Receptor (GPR10), Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR

Gene ID: 246075

NCBI Accession: NM_004248

UniProt: Q64121

Pathways: Feeding Behaviour