PRLHR antibody (3rd Intracellular Loop)

Catalog No. ABIN7043562

The Rabbit Polyclonal anti-PRLHR antibody (ABIN7043562) specifically detects PRLHR in WB. The antibody is reactive with Rat samples.

Quick Overview for PRLHR antibody (3rd Intracellular Loop) (ABIN7043562)

Target: PRLHR (Prolactin Releasing Hormone Receptor (PRLHR))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRLHR antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRLHR Antibody

Binding Specificity: 3rd Intracellular Loop, AA 247-260

Purpose: A Rabbit Polyclonal Antibody to Prolactin-Releasing Peptide Receptor

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-PrRP Receptor (GPR10) Antibody is directed against an epitope of the rat Prolactin-releasing peptide receptor. Anti-PrRP Receptor (GPR10) Antibody (ABIN7043562, ABIN7045116 and ABIN7045117) can be used in western blot analysis. It has been designed to recognize PrRPR from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RVSVKLRNRVVPGS, corresponding to amino acid residues 247-260 of rat PRLHR

Isotype: IgG

Application Details

Application Notes:

WB: 1:1000

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236617)

Blocking Peptide: (ABIN7236617)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for PRLHR

Target: PRLHR (Prolactin Releasing Hormone Receptor (PRLHR))

Alternative Name: PRLHR

Background:

Synonyms: Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR

Description: Prolactin-releasing peptide (PrRP) a bioactive peptide, was originally identified as an endogenous ligand of the orphan human G-protein coupled receptor, seven transmembrane domain receptor, GPR10 (hGR3, also called PrRP receptor). Like the majority of orphan receptors, PrRP receptor shows a low level of sequence homology with known GPCRs. Notwithstanding, a certain degree of homology has been reported to members of the neuropeptide Y (NPY) family of receptors. Neuropeptide Y is a 36- residue C-terminally amidated polypeptide hormone and neurotransmitter with the presence of an α-helix involving the 20 C-terminal residues, whereas the N-terminus part seems more flexible and is influenced by the medium environment1. It has been recently demonstrated that the PrRP receptor may modulate neurotransmission specifically at glutamatergic synapses through the interaction with the same PDZ domain proteins that interact with AMPA receptors2.Binding of PrRP to the receptor results in activation of extracellular signal-related kinase (ERK) in a mainly pertussis toxin sensitive manner, suggesting coupling to Gi/o proteins.PrRP receptor is expressed in the central nervous system with highest levels in the pituitary. Expression has also been detected in the cerebellum, brainstem, hypothalamus, thalamus, nucleus tractus solitaries, adrenal medulla and spinal cord in rat3.PrRP is implicated in the regulation of the hypothalamic-pituitary-adrenal axis, blood pressure and nociception4,5. Genetic studies in rodents indicate that lack of PrRP receptor leads to hyperphagia, obesity and dyslipidemia4. In humans, genetic variations in PrRP receptor are associated with lowered blood pressure5.

Gene ID: 246075

NCBI Accession: NM_004248

UniProt: Q64121

Pathways: Feeding Behaviour