PICK1 antibody (Intracellular)

Catalog No. ABIN7043566

This Rabbit Polyclonal antibody specifically detects PICK1 in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for PICK1 antibody (Intracellular) (ABIN7043566)

Target: PICK1 (Protein Interacting With Protein Kinase C, alpha 1 (PICK1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PICK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PICK1 Antibody

Binding Specificity: AA 305-318, Intracellular

Purpose: A Rabbit Polyclonal Antibody to PICK1

Specificity: Intracellular, α-helix domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-PICK1 Antibody is directed against an epitope of rat PICK1. Anti-PICK1 Antibody (ABIN7043566, ABIN7045156 and ABIN7045157) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Protein interacting with C kinase 1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RARFSQMRKDVLEK, corresponding to amino acid residues 305-318 of rat PICK1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236633)

Blocking Peptide: (ABIN7236633)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PICK1

Target: PICK1 (Protein Interacting With Protein Kinase C, alpha 1 (PICK1))

Alternative Name: PICK1

Background: PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein interacting with PRKCA1, Protein kinase C-alpha-binding protein,PICK1 interacts with the PDZ motifs of several proteins, including: protein kinase C α (PKC α), glutamate receptors (GluRs), prolactin-releasing peptide (PrRP), acid-sensing ion channel (ASICs), dopamine transporter (DAT), Karlirin 7, ephrin type-B receptor 2 (EphB2),ErbB2 / Her-2, TIS21, coxsackie virus and adenovirus receptor (CXADR), and UNC5H.The proteins interacting with PICK1 have roles in synaptic plasticity, neuronal cell morphology, and mitochondrial-dependent apoptosis. In most of the cases, PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory1. PICK1 is widely expressed in the brain, testis, heart, lung, liver, kidney and muscle2.Its abnormal expression in brain has been linked to drug abuse and dependence, schizophrenia, and psychosis. Recent evidence suggest that PICK1 may affect human cancer development3,4. PICK1 has also been reported to be expressed in insulin-producing pancreatic beta-cells where it may play a role in the neuroendocrine system5.

Alternative names: PICK1, PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein interacting with PRKCA1, Protein kinase C-alpha-binding protein

Gene ID: 84591

NCBI Accession: NM_012407

UniProt: Q9EP80