PACSIN1 antibody (Intracellular)

Catalog No. ABIN7043567

The Rabbit Polyclonal anti-PACSIN1 antibody has been validated for WB and IHC. It is suitable to detect PACSIN1 in samples from Rat.

Quick Overview for PACSIN1 antibody (Intracellular) (ABIN7043567)

Target: PACSIN1 (Protein Kinase C and Casein Kinase Substrate in Neurons 1 (PACSIN1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PACSIN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PACSIN1 Antibody

Binding Specificity: AA 301-315, Intracellular

Purpose: A Rabbit Polyclonal Antibody to PACSIN1

Specificity: Intracellular

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: human - 13,15 amino acid residues identical,Mouse - 14

Characteristics: Anti-PACSIN1 Antibody (ABIN7043567, ABIN7044536 and ABIN7044537) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize PACSIN1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EWNPDLPHTAAKKEK corresponding to amino acid residues 301-315 of rat PACSIN1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200-1:600

Comment:

Negative Control: (ABIN7236636)

Blocking Peptide: (ABIN7236636)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PACSIN1

Target: PACSIN1 (Protein Kinase C and Casein Kinase Substrate in Neurons 1 (PACSIN1))

Alternative Name: PACSIN1

Background: Syndapin-1, Synaptic, Dynamin-associated protein I, Protein kinase C and casein kinase substrate in neurons protein 1,The protein kinase C and casein kinase substrate in neurons (PACSIN), also known as syndapin, is an F-BAR (elongated BAR) and SH3 (src homology-3) domain-containing protein that is capable of remodeling the plasma membrane and mediating protein-protein interactions.Three different PACSIN variants are identified, which are differentially expressed: PACSIN I is the neuronal-specific isoform, PACSIN II is ubiquitously expressed and PACSIN III is expressed mainly in skeletal muscles and heart1.PACSINs interact with the large GTPase dynamin and several other proteins implicated in vesicle trafficking, PACSIN-dynamin complexes play an important role in vesicle fission at different donor membranes, including the plasma membrane (endocytosis) and Golgi membranes. In addition, PACSINs are implicated in later steps of vesicle cycling in neuronal and non-neuronal cells. PACSINs also engage in additional interactions with molecules involved in several signal transduction pathways, producing crosstalk at the interface between membrane trafficking and the cytoskeleton. Given the distinct expression patterns of the different PACSINs and their splice forms, these proteins could have isoform-specific functions2.

Alternative names: Syndapin-1, Synaptic, dynamin-associated protein I, Protein kinase C and casein kinase substrate in neurons protein 1

Gene ID: 29704

NCBI Accession: NM_001199583

UniProt: Q9Z0W5