QRFPR antibody (Extracellular)

Catalog No. ABIN7043594

The Rabbit Polyclonal anti-QRFPR antibody has been validated for WB. It is suitable to detect QRFPR in samples from Rat.

Quick Overview for QRFPR antibody (Extracellular) (ABIN7043594)

Target: QRFPR (Pyroglutamylated RFamide Peptide Receptor (QRFPR))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This QRFPR antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-QRFPR Antibody

Binding Specificity: AA 18-32, Extracellular

Purpose: A Rabbit Polyclonal Antibody to QRFP Receptor

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,17 amino acid residues identical

Characteristics: Anti-QRFPR/GPR103 (extracellular) Antibody (ABIN7043594, ABIN7044421 and ABIN7044422) is a highly specific antibody directed against an epitope of the rat QRFP receptor. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the receptor in living cells. It has been designed to recognize GPCR GPR103 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HNLTREQFIHRYGLR, corresponding to amino acid residues 18-32 of rat QRFP receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: (ABIN7236703)

Blocking Peptide: (ABIN7236703)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for QRFPR

Target: QRFPR (Pyroglutamylated RFamide Peptide Receptor (QRFPR))

Alternative Name: QRFPR

Background: Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27,QRFP is a 26-amino acid peptide (QRFP-26) that, widely distributed among vertebrates, is a member of the RF amide peptide family harboring the arginine-phenylalanine-amide (Arg-Phe-NH2) sequence motif in their carboxyl terminal. It is the cognate ligand of the human orphan receptor GPR103, a G protein-coupled receptor. Like other members of the super family, QRFP receptor has an extracellular N-terminal tail and intracellular C-terminus.Its QRFP N-terminus binds with high affinity to GPR103 and has the efficacy to inhibit cAMP synthesis. QRFP has been shown to affect locomotor activity, energy homeostasis, reproduction, bone formation, blood pressure, hormone regulation, nociception, and appetite stimulation.QRFP expressing neurons have shown to be expressed in various hypothalamic nuclei including the ventromedial hypothalamic nucleus, the lateral hypothalamic area, retrochiasmatic area and the arcuate nucleus1,2. The receptor is detected in the CNS and in peripheral tissues, notably in bone, heart, kidney, pancreas and testis.Increasing data suggest the ligand/receptor system plays an important role in the regulation of food intake and glucose homeostasis1.

Alternative names: QRFPR/GPR103, Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27

Gene ID: 310327

NCBI Accession: NM_198179

UniProt: P83858