RIC3 antibody (Intracellular)

Catalog No. ABIN7043599

This Rabbit Polyclonal antibody specifically detects RIC3 in IHC, WB and IF. It exhibits reactivity toward Mouse.

Quick Overview for RIC3 antibody (Intracellular) (ABIN7043599)

Target: RIC3 (Resistance To Inhibitors of Cholinesterase 3 Homolog (RIC3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIC3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-RIC3 Antibody

Binding Specificity: AA 161-174, Intracellular

Purpose: A Rabbit Polyclonal Antibody to RIC-3

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-RIC3 Antibody (ABIN7043599, ABIN7044633 and ABIN7044634) is a highly selective antibody directed against an epitope of mouse protein . The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize RIC3 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EKLINRVGPNGESR, corresponding to amino acid residues 161-174 of mouse RIC3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236722)

Blocking Peptide: (ABIN7236722)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for RIC3

Target: RIC3 (Resistance To Inhibitors of Cholinesterase 3 Homolog (RIC3))

Alternative Name: RIC3

Background: Protein RIC-3, RIC3 acetylcholine receptor chaperone, Resistant to inhibitor of cholinesterase 3,RIC-3 (Resistance to inhibitors of cholinesterase 3) is a conserved endoplasmic reticulum-resident protein that is known to be a chaperone for the nicotinic acetylcholine receptors (nAChR) and for the serotonin (5-HT3) channels1.RIC-3 affects the maturation of multiple nAChRs which has been shown to facilitate nAChR assembly and trafficking3,4.Multiple transcripts of RIC-3 displaying differential expression are detected in humans. The full-length transcript includes two transmembrane domains followed by a coiled-coil domain, a nonconserved N-terminus and C-terminus domain, which includes a second coiled-coil domain. The short isoform lacks the coiled-coil domains and is very conserved in mammals1,2,4.

Alternative names: RIC3, Protein RIC-3, RIC3 acetylcholine receptor chaperone, Resistant to inhibitor of cholinesterase 3

Gene ID: 320360

NCBI Accession: NM_024557

UniProt: Q8BPM6