ROBO1 antibody (Extracellular)

Catalog No. ABIN7043603

Product Details anti-ROBO1 Antibody

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Binding Specificity: AA 491-506, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROBO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Robo1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: Anti-Robo1 (extracellular) Antibody (ABIN7043603, ABIN7044744 and ABIN7044745) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize Robo1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DGVLVSTQDSRIKQLE, corresponding to amino acid residues 491 - 506 of human Robo1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236733)

Blocking Peptide: (ABIN7236733)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ROBO1

Target: ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))

Alternative Name: ROBO1 (ROBO1 Products)

Background: Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1,Roundabout (Robo) receptors are a conserved family of four single pass transmembrane proteins1. All members have multiple immunoglobulin-like domains in the extracellular N-terminal region, three extracellular fibronectin type II domains, and cytoplasmic conserved domains. Robos also undergo preoteolytic processing, important for their signaling process2. They serve as receptors for Slit proteins, where upon activation lead to pleiotropic effects including axonal guidance1, axonal repulsion3,4, and neural migration5.Slit/Robo signaling mainly prevents axons from migrating to inappropriate locations during the assembly of the nervous system6.Robos are mainly expressed in the nervous system, but are also detected in vascular endothelial and muscle cells7. In non-neuronal tissues, Slit/Robo proteins are key oncogenes and tumor suppressors in various types of cancers. Robo1 expression is elevated in breast cancer and colorectal cancer, and has an overall oncogenic role1.

Alternative names: Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1

Gene ID: 6091

NCBI Accession: NM_002941

UniProt: Q9Y6N7

Pathways: Positive Regulation of Endopeptidase Activity