SCTR antibody (Extracellular, N-Term)

Catalog No. ABIN7886420

The Rabbit Polyclonal anti-SCTR antibody (ABIN7886420) specifically detects SCTR in WB, IHC and IF. The antibody is reactive with Rat samples.

Quick Overview for SCTR antibody (Extracellular, N-Term) (ABIN7886420)

Target: SCTR (Secretin Receptor (SCTR))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCTR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SCTR Antibody

Binding Specificity: AA 129-143, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Secretin Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 15 amino acid residues identical,Mouse - 14, human - 12

Characteristics: Anti-Secretin Receptor (extracellular) Antibody (ABIN7043607, ABIN7044414 and ABIN7044415) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SCTR from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: Peptide (C)NSFNERRHAYLLKLK, corresponding to amino acid residues 129 - 143 of rat Secretin Receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7236749)

Blocking Peptide: (ABIN7236749)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for SCTR

Target: SCTR (Secretin Receptor (SCTR))

Alternative Name: SCTR

Background:

Synonyms: SCTR

Description: Secretin receptor, SCTR, binds Secretin, a hormone that affects water homeostasis throughout the body and specifically in the duodenum of the small intestine.SCTR is ubiquitously expressed throughout the body. It belongs to class B1 of G-protein coupled receptors, along with vasoactive intestinal polypeptide (VIP) receptor. Like all G-protein coupled receptors, it is composed of seven highly conserved transmembrane domains1. The amino terminus of Secretin receptor plays a key role in agonist recognition and responsiveness2.The binding of SCTR to Secretin initiates a signaling cascade that stimulates bicarbonate secretion which in turn leads to neutralization of gastric H+. This process plays an essential role in fat digestion by creating a more neutral ( pH 6 to 8) environment. Secretin levels are regulated by H+ and fatty acids levels in the duodenum and hence the secretion regulation is tightly bound to its activity3.In addition, SCTR-Secretin interaction plays a major role in the regulation of the secretory activity in the heart, liver and the central nervous system (CNS)3.

Gene ID: 6344

NCBI Accession: NM_002980

UniProt: P23811