Serotonin Receptor 3B antibody (Extracellular) (Atto 488)

Catalog No. ABIN7043623

Product Details anti-Serotonin Receptor 3B Antibody (Atto 488)

Target: Serotonin Receptor 3B (HTR3B)

Binding Specificity: AA 213-226, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3B antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3B Conjugated to the Fluorescent Dye ATTO-488

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identicalHuman- not recommended for use with human samples

Characteristics: Anti-5HT3B Receptor (HTR3B) (extracellular) Antibody (ABIN7043622 and ABIN7045285)) is a highly specific antibody directed against an epitope of rat Serotonin receptor 3B. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect 5-HT3B in living cells. It has been designed to recognize 5-HT3B from rat and mouse samples. The antibody is unlikely to recognize the receptor from human samples. \nAnti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody (#ABIN7043623) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-5HT3B Receptor (HTR3B) (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HIRQSSAGDFAQIR, corresponding to amino acid residues 213-226 of rat 5-Hydroxytryptamine receptor 3B

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: RIC-001-AG

Blocking Peptide: BLP-SR032

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Serotonin Receptor 3B

Target: Serotonin Receptor 3B (HTR3B)

Alternative Name: HTR3B (HTR3B Products)

Background: 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B,5-Hydroxytryptamine receptor 3B (5-HT3B, HTR3B) belongs to the super-family of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the super-family of G-protein coupled receptors.The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified and labeled 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types.In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The localization of 5-HT3B subunits is somewhat controversial, some studies show that its expression is restricted to the peripheral nervous system and others show that it is also detected in the brain and in hippocampal neurons1,4-7.5-HT3 receptors have become important targets for which to develop treatments for irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2.

Alternative names: 5HT3B Receptor (HTR3B), 5-Hydroxytryptamine receptor 3B, Serotonin receptor 3B

Gene ID: 58963

NCBI Accession: NM_006028

UniProt: Q9JJ16

Pathways: Synaptic Membrane