SLITRK1 antibody (Extracellular)

Catalog No. ABIN7043629

This Rabbit Polyclonal antibody specifically detects SLITRK1 in WB and IHC. It exhibits reactivity toward Mouse.

Quick Overview for SLITRK1 antibody (Extracellular) (ABIN7043629)

Target: SLITRK1 (SLIT and NTRK-Like Family, Member 1 (SLITRK1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLITRK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Grade: KO Validated

Product Details anti-SLITRK1 Antibody

Binding Specificity: AA 383-397, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Slitrk1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human - identical, rat - 14,15 amino acid residues identical

Characteristics: Anti-Slitrk1 (extracellular) Antibody (ABIN7043629, ABIN7045304 and ABIN7045305) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize Slitrk1 from mouse, human, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RDNKIHSIRKSHFVD, corresponding to amino acid residues 383-397 of mouse Slitrk1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200-1:300

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236807)

Blocking Peptide: (ABIN7236807)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLITRK1

Target: SLITRK1 (SLIT and NTRK-Like Family, Member 1 (SLITRK1))

Alternative Name: SLITRK1

Background: SLIT and NTRK-like family member 1, Leucine-rich repeat-containing protein 12, LRRC12,Neurites are key morphological features of neurons, and are classified into axons and dendrites. Leucine-rich repeat (LRR) domains are often found in neurite development-controlling proteins. Slitrk proteins are leucine-rich repeat containing transmembrane proteins, parts of which are similar to Slit and neurotrophin receptor.All six members of the Slitrk family, including Slitrk1, contain putative hydrophobic signal sequences and membrane-spanning regions. Slitrk1 is strongly expressed in the subventricular zone of the cerebral cortex, the pyramidal cell layer of the hippocampus and in the thalamus and hypothalamus1.Slitrk1, can regulate synapse formation between hippocampal neurons. Slitrk1 is enriched in postsynaptic fractions and is localized to excitatory synapses. Overexpression of Slitrk1 in hippocampal neurons increases the number of synaptic contacts on these neurons. Furthermore, decreased expression of Slitrk1 in hippocampal neurons leads to a reduction in the number of excitatory, but not inhibitory, synapses formed in hippocampal neuron cultures. In addition, different leucine rich repeat domains of the extracellular region of Slitrk1 are necessary to mediate interactions with Slitrk binding partners of the LAR receptor protein tyrosine phosphatase family, and to promote dimerization of Slitrk1. In addition, Slitrk1 binds with PSD-95 through its intracellular tail. Thus, through PSD-95, Slitrk1 regulates signaling of NMDA and AMPA receptors2.

Alternative names: Slitrk1, SLIT and NTRK-like family member 1, Leucine-rich repeat-containing protein 12, LRRC12

Gene ID: 76965

NCBI Accession: NM_052910

UniProt: Q810C1