SCN11A antibody (Intracellular)

Catalog No. ABIN7043657

The Rabbit Polyclonal anti-SCN11A antibody is suitable to detect SCN11A in samples from Rat. It has been validated for IHC, WB, IF and IC.

Quick Overview for SCN11A antibody (Intracellular) (ABIN7043657)

Target: SCN11A (Sodium Channel, Voltage-Gated, Type XI, alpha Subunit (SCN11A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCN11A antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-SCN11A Antibody

Binding Specificity: AA 1748-1765, Intracellular

Purpose: A Rabbit Polyclonal Antibody to SCN11A (NaV1.9) Channel

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 18 amino acid residues identical, human - 14,Mouse - 15

Characteristics: Anti-SCN11A (NaV1.9) Antibody (ABIN7043657, ABIN7045241 and ABIN7045242) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize NaV1.9 channel from rat, human, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: (ABIN7236878)

Blocking Peptide: (ABIN7236878)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SCN11A

Target: SCN11A (Sodium Channel, Voltage-Gated, Type XI, alpha Subunit (SCN11A))

Alternative Name: SCN11A

Background: PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha,Voltage-gated Na+ channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-1.9.2-3 Expression of the α subunit is developmentally and tissue specific.The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive and TTX-resistant channels.4-5 TTX-resistant channels have been suggested to play an important role in nociceptive transmission. Two TTX-resistant NaV channels are expressed in dorsal root ganglion (DRG) neurons, NaV1.8 and NaV1.9. The NaV1.9 channel (also called SCN11A, NAN or SNS2) is preferentially expressed in small-diameter DRG neurons.4-5Recently, it was shown that BDNF activates NaV1.9 channels in the hippocampus suggesting that its expression is not restricted to DRG alone.6

Alternative names: SCN11A (NaV1.9), PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha

Gene ID: 29701

NCBI Accession: NM_014139

UniProt: O88457