SLC1A3 antibody (Extracellular) (FITC)

Catalog No. ABIN7043663

Product Details anti-SLC1A3 Antibody (FITC)

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Binding Specificity: AA 188-200, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC1A3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 1 (EAAT1, GLAST) Conjugated to the Fluorescent Dye FITC

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human 12,13 amino acid residues identical

Characteristics: Anti-EAAT1 (GLAST) (extracellular) Antibody (ABIN7043662, ABIN7044352 and ABIN7044353) is a highly specific antibody directed against an epitope of rat Excitatory amino acid transporter 1. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize EAAT1 from rat, human and mouse samples. \nAnti-EAAT1 (GLAST) (extracellular)-FITC Antibody (ABIN7043662, ABIN7044352 and ABIN7044353)-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as live cell direct flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KQFKTSYEKRSFK, corresponding to amino acids 188-200 of rat EAAT1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236898)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC1A3

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Alternative Name: SLC1A3 (SLC1A3 Products)

Background: Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, SLC1A3,Glutamate transporters play important roles in the termination of excitatory neurotransmission and in providing cells with glutamate for metabolic purposes.SLC1A3 (EAAT1) belongs to The SLC1 protein family which contains a range of human and prokaryotic glutamate and aspartate transporters. EAAT1 transports the acidic amino acids glutamate and aspartate against a concentration gradient generating a net movement of substrate and charge across the membrane. Glutamate transport by EAAT1 is coupled to the co-transport of three Na+ ions and one H+ ion, and the counter transport of one K+ ion1.EAAT1 is mainly expressed in astrocytes but can also be found in oligodendrocytes. EAAT1, together with EAAT2, are responsible for the bulk of glutamate uptake in the brain. They have several important roles, including the termination of the synaptic effects of glutamate, prevention of potentially toxic accumulation of extracellular glutamate, supply of glutamate to the astrocyte for synthesis of glutamine, which is involved in ammonia detoxification and in the glutamine-glutamate cycle and signaling of the metabolic needs of nearby neurons and activation of glycolysis in astrocytes2.EAAT1 has been implicated in the pathogenesis of episodic ataxia, a rare neurological disorder characterized by neonatal muscle stiffness and an exaggerated startle reflex induced by noise or touch3.

Alternative names: EAAT1 (GLAST), Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, SLC1A3

Gene ID: 29483

NCBI Accession: NM_004172

UniProt: P24942

Pathways: Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport