SLC1A3 antibody (C-Term)

Catalog No. ABIN3044001

Cited in 2+ publications. The Rabbit Polyclonal anti-SLC1A3 antibody (ABIN3044001) specifically detects SLC1A3 in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for SLC1A3 antibody (C-Term) (ABIN3044001)

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC1A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SLC1A3 Antibody

Binding Specificity: AA 519-537, C-Term

Purpose: Anti-EAAT1/SLC1A3 Antibody

Sequence: MKKPYQLIAQ DNETEKPID

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-EAAT1/SLC1A3 Antibody (ABIN3044001). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT1, different from the related rat and mouse sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μ/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-SLC1A3 antibody (ABIN3044001)

Ho, Hartley, Flaherty, Rajarajan, Abdelaal, Obiorah, Barretto, Muhammad, Phatnani, Akbarian, Brennand: "Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes." in: Stem cell reports, Vol. 9, Issue 2, pp. 615-628, (2018) (PubMed).

Ding, Zhang, Liu, Zhang, Ma, Bruce, Zhang: "Tumor necrosis factor-? promotes the expression of excitatory amino-acid transporter 2 in astrocytes: Optimal concentration and incubation time." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1909-1913, (2014) (PubMed).

Target Details for SLC1A3

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Alternative Name: SLC1A3

Background:

Synonyms: Excitatory amino acid transporter 1,Sodium-dependent glutamate/aspartate transporter 1,GLAST-1,Solute carrier family 1 member 3,SLC1A3,EAAT1, GLAST, GLAST1,

Tissue Specificity: Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Background: Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. SLC1A3 is mapped to chromosome 5p13.2 by fluorescence in situ hybridization (FISH). This gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. This gene acts as a symport by cotransporting sodium.

Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Molecular Weight: 274 kDa

UniProt: P43003

Pathways: Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport