SLC18A2 antibody (AA 52-64)

Catalog No. ABIN7043688

Product Details anti-SLC18A2 Antibody

Target: SLC18A2 (Solute Carrier Family 18 (Vesicular Monoamine Transporter), Member 2 (SLC18A2))

Binding Specificity: AA 52-64

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC18A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Vesicular Monoamine Transporter 2

Specificity: 1st luminal loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,human - 12,13 amino acid residues identical

Characteristics: Anti-VMAT2 Antibody (ABIN7043688, ABIN7044610 and ABIN7044611) is a highly specific antibody directed against an epitope of the rat vesicular monoamine transporter 2. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize VMAT2 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KHEKNSTEIQT(A)R, corresponding to amino acid residues 52-64 of rat VMAT2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7236979)

Blocking Peptide: (ABIN7236979)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC18A2

Target: SLC18A2 (Solute Carrier Family 18 (Vesicular Monoamine Transporter), Member 2 (SLC18A2))

Alternative Name: SLC18A2 (SLC18A2 Products)

Background: Vesicular monoamine transporter 2, SLC18A2, SVAT, VAT2,Vesicular monoamine transporters (VMATs) are essential for proper monoaminergic neurotransmission, which requires the sequestration of the transmitter into synaptic vesicles by VMAT for subsequent Ca2+-stimulated exocytotic release. VMATs contain 12 transmembrane spanning domains, with cytosolic C- and N-terminals and large glycosylated intravesicular loops. In mammals, there are two VMAT isoforms: VMAT1 (SLC18A1) and VMAT2 (SLC18A2)1.VMATs primarily transport monoamines (dopamine, serotonin, norepinephrine and histamine), but also sequester toxicants into vesicles, shunting them away from cytosolic sites of action. In the central nervous system, VMAT2 is the only transporter that moves cytoplasmic dopamine (DA) into synaptic vesicles for storage and subsequent exocytotic release2.VMAT2 is expressed in monoaminergic neurons of the central nervous system (CNS) and sympathetic postganglionic neurons. VMAT2 is widely expressed during embryonic development3. Homozygous knock-out of VMAT2 in mice is lethal and heterozygous knock-out of VMAT2 in mice shows a clear gene dosage effect, with half the amount of dopamine, norepinephrine, and serotonin content in brain4. In addition VMAT2 plays a role in neuroprotection and molecules that interact with VMAT2 may have value as treatments for diseases such as Parkinson's disease5.

Alternative names: VMAT2, Vesicular monoamine transporter 2, SLC18A2, SVAT, VAT2

Gene ID: 25549

NCBI Accession: NM_003054

UniProt: Q01827