SLC29A3 antibody (Intracellular)

Catalog No. ABIN7043712

Product Details anti-SLC29A3 Antibody

Target: SLC29A3 (Solute Carrier Family 29 Member 3 (SLC29A3))

Binding Specificity: AA 37-49, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC29A3 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Equilibrative Nucleoside Transporter 3 (SLC29A3)

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 10,13 amino acid residues identical

Characteristics: Anti-ENT3 (SLC29A3) Antibody (ABIN7043712, ABIN7044802 and ABIN7044803)) is a highly specific antibody directed against an epitope of the rat equilibrative nucleoside transporter 3. The antibody can be used in western blot analysis. It has been designed to recognize ENT3 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DYPAPGLQRPEDR, corresponding to amino acid residues 37-49 of rat ENT3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NT053

Blocking Peptide: BLP-NT053

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC29A3

Target: SLC29A3 (Solute Carrier Family 29 Member 3 (SLC29A3))

Alternative Name: SLC29A3 (SLC29A3 Products)

Background: Equilibrative nucleoside transporter 3,Equilibrative nucleoside transporter 3 (ENT3) is responsible for transporting nucleotide synthesis precursors, such as hydrophilic nucleosides and nucleobases within cells1. ENT3 structure includes 11 transmembrane domains and a 51-residue hydrophilic N-terminal region containing two dileucine motifs that are characteristic of endosomal/lysosomal targeting sequences1,2.ENT3 is encoded by the SLC29A3 gene and is expressed in several mouse and human tissues such as placenta, heart, neurons, and astrocytes. In addition, human ENT3 is predominantly expressed in mitochondria in human hepatocytes. Human ENTs are high-capacity transporters, bidirectional, Na+-independent with a wide substrate selectivity1,2. The human ENT family consists of four members: ENT1- ENT4. Nucleoside transport function of ENT3 is maximal at pH 5.5 suggesting that the protein is localized to acidic intracellular compartments, probably lysosomes1,2.Studies show that mutations in human ENT3 can cause various clinical disorders including H syndrome, pigmented hypertrichosis with insulin-dependent diabetes mellitus syndrome, lysosomal storage-like disorders and mitochondrial disorders1,2.

Alternative names: ENT3 (SLC29A3), Equilibrative nucleoside transporter 3

Gene ID: 353307

NCBI Accession: NM_018344

UniProt: Q80WK7