SLC3A1 antibody (Extracellular)

Catalog No. ABIN7043713

This Rabbit Polyclonal antibody specifically detects SLC3A1 in WB. It exhibits reactivity toward Rat.

Quick Overview for SLC3A1 antibody (Extracellular) (ABIN7043713)

Target: SLC3A1 (Solute Carrier Family 3 Member 1 (SLC3A1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC3A1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SLC3A1 Antibody

Binding Specificity: AA 277-291, Extracellular

Purpose: A Rabbit Polyclonal Antibody to SLC3A1

Specificity: Extracellular, C-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14,15 amino acid residues identical

Characteristics: Anti-SLC3A1 (extracellular) Antibody (ABIN7043713, ABIN7044829 and ABIN7044830) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize SLC3A1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEQPDLNFRNPAVQE, corresponding to amino acid residues 277-291 of rat SLC3A1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7237055)

Blocking Peptide: (ABIN7237055)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC3A1

Target: SLC3A1 (Solute Carrier Family 3 Member 1 (SLC3A1))

Alternative Name: SLC3A1

Background: Neutral and basic amino acid transport protein rBAT, Solute carrier family 3 (cystine, dibasic and neutral amino acid transporters) member 1, B(0,+)-type amino acid transport protein, NBAT, Amino acid transporter 1, ATR1,The heteromeric amino acid transporter (HAT) family is one of the major amino acid transporter families responsible for cellular uptake and epithelial transport. The uptake of cysteine mainly depends on HATs. HATs are localized to the plasma membrane and form heterodimers composed of a 12-membrane spanning light chain (SLC7) and a single membrane spanning heavy chain, SLC3. SLC7 catalyzes transport functions while SLC3 is essential for plasma membrane localization and stabilization of the light chains. The SLC3A1 is mainly expressed in the apical plasma membrane in the epithelial cells of intestinal mucosa and the renal proximal tubules1,2. Two heavy chains, SLC3A1 and SLC3A2 are covalently bound to the light chains through a disulfide bridge. SLC3A1 has been known to form a heterodimer only with SLC7A9, a family member of light chain1,2.Type 1 Cystinuria is associated with mutations in SLC3A1, an inherited disease characterized by the formation of cystine calculi in the kidneys, ureters, and bladder3.

Alternative names: SLC3A1, Neutral and basic amino acid transport protein rBAT, Solute carrier family 3 (cystine, dibasic and neutral amino acid transporters) member 1, B(0,+)-type amino acid transport protein, NBAT, Amino acid transporter 1, ATR1

Gene ID: 29484

NCBI Accession: NM_000341

UniProt: Q64319