Slc30a3 antibody (AA 210-223)

Catalog No. ABIN7043715

Product Details anti-Slc30a3 Antibody

Target: Slc30a3 (Solute Carrier Family 30 (Zinc Transporter), Member 3 (Slc30a3))

Binding Specificity: AA 210-223

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Slc30a3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Zinc Transporter 3

Specificity: 2nd intracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical,Mouse - 12, human - 11

Characteristics: Anti-ZnT3 (SLC30A3) Antibody is directed against an epitope of the rat zinc transporter 3. Anti-ZnT3 (SLC30A3) Antibody (ABIN7043715, ABIN7045356 and ABIN7045357) can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZnT3 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RGAEYAPLEEGHGH, corresponding to amino acid residues 210-223 of rat ZnT3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:200-1:500

Comment:

Negative Control: (ABIN7237063)

Blocking Peptide: (ABIN7237063)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Slc30a3

Target: Slc30a3 (Solute Carrier Family 30 (Zinc Transporter), Member 3 (Slc30a3))

Alternative Name: SLC30A3 (Slc30a3 Products)

Background: Zinc transporter 3,ZnT3 is a member of the SLC30 family of zinc transporters which promotes the influx of zinc ions into synaptic vesicles of glutamatergic neurons from the cytoplasm, intracellular organelles, or to the outside of the cell. It is responsible for maintaining high intravesicular zinc content.ZnT3 is encoded by the Slc30a3 gene and is selectively located on the vesicles of zinc-secreting neurons. ZnT3 is also present in regions of the brain such as the hippocampus and neocortex1. Ionic zinc is found ubiquitously throughout synapses of the mammalian central nervous system, where it plays fundamental roles in synaptic function and plasticity2.ZnT3 structure is predicted to contain six transmembrane spanning domains that form a pore lined with a histidine-rich loop3.Knockout of ZnT3 leads to the absence of histochemically reactive zinc in the terminals of zincergic neurons. A loss of ZnT3 might lead to disturbances in brain functions such as memory function, emotions, behavior, motivation, and in the processing of sensory information3,4. Studies have shown that genetic absence of ZnT3 may be involved in the synaptic and memory deficits of Alzheimer's disease1. Another study showed that a knockout mouse has a tendency for seizure5.

Alternative names: ZnT3 (SLC30A3), Zinc transporter 3

Gene ID: 366568

NCBI Accession: NM_001318949

UniProt: Q6QIX3

Pathways: Transition Metal Ion Homeostasis