SLC47A2 antibody (Extracellular)

Catalog No. ABIN7043733

This anti-SLC47A2 antibody is a Rabbit Polyclonal antibody detecting SLC47A2 in WB. Suitable for Mouse.

Quick Overview for SLC47A2 antibody (Extracellular) (ABIN7043733)

Target: SLC47A2 (Solute Carrier Family 47, Member 2 (SLC47A2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC47A2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SLC47A2 Antibody

Binding Specificity: AA 481-496, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Multidrug and Toxin Extrusion Protein 2 (MATE2, SLC47A2)

Specificity: 6th intracellular loop

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: Rat - 14,16 amino acid residues identical, human - not recommended for use with human samples

Characteristics: Anti-SLC47A2 Antibody is directed against an epitope of mouse Multidrug and toxin extrusion protein 2 (MATE2, SLC47A2). Anti-SLC47A2 Antibody (ABIN7043733, ABIN7044843 and ABIN7044844) can be used in western blot analysis. It has been designed to recognize SLC47A2 from rat and mouse samples. The antibody will not recognize SLC47A2 from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RAGLKSTKELIPTPAD, corresponding to amino acid residues 481-496 of mouse SLC47A2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237126)

Blocking Peptide: (ABIN7237126)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC47A2

Target: SLC47A2 (Solute Carrier Family 47, Member 2 (SLC47A2))

Alternative Name: SLC47A2

Background: MATE2, Multidrug and toxin extrusion protein 2, H+/organic cation antiporter kidney-specific,Solute carriers control the efflux and uptake of crucial compounds such as sugars, amino acids and ions in cells and organelles1. Multidrug and toxin extrusion proteins, also known as MATEs are responsible for the efflux of organic cations, endogenous compounds and drugs from cells. The driving force for this transport is derived from an oppositely directed proton gradient thus making MATE transporters secondary active transporters. MATEs work in concert with OCTs (Organic cation transporters). While the latter are located in the basolateral cell membrane of proximal tubules cells MATEs are expressed on the apical membrane2. MATEs can also transport zwitterions and anionic compounds in addition to cations.MATE2 carrier is part of the SLC47 family encoded by the SLC47A2 gene, predominantly expressed in apical brush border membranes of the proximal tubules in kidneys. It exhibits proton gradient dependent secretion of cationic drugs across the membrane. It is predicted to have 12 transmembrane helices similar to other MATE family members but also an additional COOH-terminal helix. Histidine and Cysteine residues in positions 382, 59 and 123 respectably are required for MATE2 proper functioning3.Metformin, a commonly used hypoglycemic agent and platinum based chemotherapeutics are mainly excreted by MATE transporters in the kidney. Changes in the expression of MATEs can dramatically influence the concentration of these substances in the body and cause serious side effects. In a recent study, pyrimethamine, an inhibitor of MATE transporters reduced metformin renal clearance by 35 % 4. Inhibition of MATE transporters by anti-neoplastic drugs can play a role in chemosensitivity of cancerous cells. MATEs are a potential target for prevention of resistance to treatment and toxicity during chemotherapy5.

Alternative names: SLC47A2, MATE2, Multidrug and toxin extrusion protein 2, H+/organic cation antiporter kidney-specific

Gene ID: 380701

NCBI Accession: NM_152908

UniProt: Q3V050