SLC7A11 antibody (Extracellular)

Catalog No. ABIN7043751

This anti-SLC7A11 antibody is a Rabbit Polyclonal antibody detecting SLC7A11 in WB, IHC, FACS, IF and LCI. Suitable for Mouse.

Quick Overview for SLC7A11 antibody (Extracellular) (ABIN7043751)

Target: SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC7A11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Product Details anti-SLC7A11 Antibody

Binding Specificity: AA 218-231, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Cystine/Glutamate Transporter (xCT)

Specificity: 3rd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 11,14 amino acid residues identical

Characteristics: Anti-xCT/SLC7A11 (extracellular) Antibody (ABIN7043751, ABIN7044835 and ABIN7044836) is a highly selective antibody directed against an epitope of the mouse cystine/glutamate transporter. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize xCT from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)THHFKDAFSGRDTS, corresponding to amino acid residues 218-231 of mouse cystine/glutamate transporter

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:800

Comment:

Negative Control: (ABIN7237192)

Blocking Peptide: (ABIN7237192)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC7A11

Target: SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))

Alternative Name: SLC7A11

Background: Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1,The SLC7 family is divided into two subgroups, the cationic amino acid transporters (the CAT family, SLC7A1-4) and the glycoprotein-associated amino acid transporters (the gpaAT family, SLC7A5-11) also called light chains or catalytic chains of the hetero(di)meric amino acid transporters (HAT). The gene SLC7A11 encodes the xCT protein (also known as Cystine/glutamate transporter).xCT, as well as other HATs, is comprised of a light chain with 12 non-glycosylated transmembrane helices and is associated with the glycosylated heavy chain 4F2hc (CD98, SLC3A2) or rBAT (SLC3A1) through a conserved disulphide bridge. The COOH-terminus of the xCT is localized intracellularly. Association of the light and heavy chains is required for surface expression of the transporter which forms the heterodimeric amino acid transport system xc-. xCT is mainly expressed macrophages, brain, retinal pigment cells, liver and kidney. xCT expression is elevated in cells requiring high glutathione synthesis, such as activated macrophages, neuronal and glial cells and other cells after glutathione depletion1.xCT operates in a Na+-independent and electroneutral mode, exchanging extracellular anionic cystine (with pH dependence) for glutamate with a stoichiometry of 1:1. The driving force for this exchange is generated by the cystine concentration gradient (intracellular reduction) and the high intracellular concentration of glutamate.xCT has been suggested as a mediator of treatment resistance in cancer patients. In tumor cells, xCT plays a crucial role in regulating intracellular levels of glutathione which has been broadly implicated in resistance to chemotherapy2.

Alternative names: xCT/SLC7A11, Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1

Gene ID: 26570

NCBI Accession: NM_014331

UniProt: Q9WTR6