Sortilin 1 antibody (Extracellular)

Catalog No. ABIN7043768

The Rabbit Polyclonal anti-Sortilin 1 antibody has been validated for WB, IHC, FACS, IF and LCI. It is suitable to detect Sortilin 1 in samples from Human.

Quick Overview for Sortilin 1 antibody (Extracellular) (ABIN7043768)

Target: Sortilin 1 (SORT1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sortilin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Grade: KO Validated

Product Details anti-Sortilin 1 Antibody

Binding Specificity: AA 320-335, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Sortilin

Specificity: Extracellular domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: Anti-Sortilin (extracellular) Antibody (ABIN7043768, ABIN7044759 and ABIN7044760) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize Sortilin from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: DKDTTRRIHVSTDQD(C) corresponding to amino acid residues 320-335 of human Sortilin

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Cited Application: IP|IHC

Negative Control: (ABIN7237255)

Blocking Peptide: (ABIN7237255)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Sortilin 1

Target: Sortilin 1 (SORT1)

Alternative Name: SORT1

Background: SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95,Sortilin is a member of the Vps10p family named for a yeast gene that is involved in trafficking between the trans-golgi network and the vacuole. In mammals sortilin has been shown to play an important role in golgi to endosome and golgi to lysosome trafficking. Sortilin is also known as neurotensin receptor 3 (NTS3), one of the receptors for the peptide neurotransmitter neurotensin that exerts several biological functions ranging from the regulation of dopamine transmission and pain in the central nervous system to its functioning as a local hormone affecting the gastrointestinal tract. In addition, sortilin has lately been identified as a co-receptor of p75NTR for the binding of the proneurotrophins proNGF and proBDNF. Moreover, sortilin expression was found to be essential for proNGF and proBDNF-induced neuronal cell death.Sortilin is a type I membrane protein with a large extracellular domain that includes 8 bacterial neuraminidase repeats (BNRs), a single transmembrane region and a short cytoplasmic tail. The protein is expressed in several tissues, most notably in the brain, spinal cord, skeletal muscle and adipocytes.Paradoxically for its role as either a neurotensin receptor or a p75NTR co-receptor for proneurotrophins, about 90 % of sortilin is retained in intracellular compartments with only about 10 % reaching the cell surface. It has been speculated, that changes in the cellular environment may increase cell surface expression of sortilin.

Alternative names: Sortilin, SORT1, Neurotensin Receptor 3, NTR3, NT3, Glycoprotein 95, Gp95

Gene ID: 6272

NCBI Accession: NM_002959

UniProt: Q99523

Pathways: Neurotrophin Signaling Pathway