STIM1 antibody (Extracellular) (Atto 550)

Catalog No. ABIN7043780

The Rabbit Polyclonal anti-STIM1 antibody has been validated for IHC, IF, FACS, IC and LCI. It is suitable to detect STIM1 in samples from Human.

Quick Overview for STIM1 antibody (Extracellular) (Atto 550) (ABIN7043780)

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STIM1 antibody is conjugated to Atto 550

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-STIM1 Antibody (Atto 550)

Binding Specificity: AA 56-69, Extracellular

Purpose: A Rabbit Polyclonal Antibody to STIM1 Conjugated to the Fluorescent Dye ATTO-550

Specificity: STIM1 expressed on the plasma membrane: Extracellular, N-terminus STIM1 expressed on the ER: Luminal, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,bovine,canis - identical

Characteristics: Anti-STIM1 (extracellular) Antibody (ABIN7043781, ABIN7044026 and ABIN7044027) is a highly specific antibody directed against an extracellular epitope of the human Stromal interaction molecule 1. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize STIM1 from human, rat, and mouse samples. \nAnti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (ABIN7043780) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:20

Application Dilutions Western blot wb: N/A

Comment:

Cited Application: FC

Negative Control: (ABIN7582042)

Blocking Peptide: (ABIN7237292)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for STIM1

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Alternative Name: STIM1

Background: Stromal interaction molecule 1, GOK,Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest.Recently, several key players of the store-operated complex have been identified.3 The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+ binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 Molecules are redistributed in punctae underneath the plasma membrane and activate SOCs.Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.5 Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed.

Alternative names: STIM1, Stromal interaction molecule 1, GOK

Gene ID: 6786

NCBI Accession: NM_003156

UniProt: Q13586

Pathways: TCR Signaling, BCR Signaling