STIM1 antibody (Extracellular)

Catalog No. ABIN7043781

Product Details anti-STIM1 Antibody

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Binding Specificity: AA 56-69, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STIM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunoprecipitation (IP), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to STIM1

Specificity: STIM1 expressed on the plasma membrane: Extracellular, N-terminus STIM1 expressed on the ER: Luminal, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,bovine,canis - identical

Characteristics: Anti-STIM1 (extracellular) Antibody (ABIN7043781, ABIN7044026 and ABIN7044027) is a highly specific antibody directed against an epitope of the human Stromal interaction molecule 1. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize STIM1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:1000

Comment:

Negative Control: (ABIN7237292)

Blocking Peptide: (ABIN7237292)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for STIM1

Target: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Alternative Name: STIM1 (STIM1 Products)

Background: Stromal interaction molecule 1, GOK,Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest.Recently, several key players of the store-operated complex have been identified.3 The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+-binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 Molecules are redistributed in punctae underneath the plasma membrane and activate SOCs.Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.5 Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed.

Alternative names: STIM1, Stromal interaction molecule 1, GOK

Gene ID: 6786

NCBI Accession: NM_003156

UniProt: Q13586

Pathways: TCR Signaling, BCR Signaling