SYN2 antibody (Intracellular)

Catalog No. ABIN7043787

Product Details anti-SYN2 Antibody

Target: SYN2 (Synapsin II (SYN2))

Binding Specificity: AA 438-450, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to Synapsin II

Specificity: Intracellular, region G

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): This antibody will recognize both canonical isoforms (also known as Synapsin IIa) and the shorter isoform (Synapsin-IIb).

Predicted Reactivity: Mouse - identical, human- 12,13 amino acid residues identical

Characteristics: Anti-Synapsin II (SYN2) Antibody (ABIN7043787, ABIN7044660 and ABIN7044661)) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Synapsin II from mouse, human, and rat samples. This antibody will recognize both canonical isoforms (also known as Synapsin IIa) and the shorter isoform (Synapsin IIb).

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SGTLKEPDSSKTP, corresponding to amino acid residues 438-450 of rat Synapsin-2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NR015

Blocking Peptide: BLP-NR015

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SYN2

Target: SYN2 (Synapsin II (SYN2))

Alternative Name: SYN2 (SYN2 Products)

Background: Synapsin-2,Synapsins are a family of ten homologous neuronal phosphoproteins evolutionarily conserved in invertebrate and vertebrate organisms. Synapsins appear to be crucial determinants for the fine-tuning of synaptic transmission and for synaptic remodeling. This is particularly evident in several paradigms of short-term plasticity (STP), which are specifically affected by mutations or malfunctioning of synapsin proteins. Notably, knockout of synapsin-2 in mice causes fully generalized forebrain seizures from the age of 2 months1.All synapsins, including synapsin-2, are composed of a highly homologous N-terminal region, and a more variable C-terminus. The N-terminal homologous region can be divided in three domains, namely domain A, B and C, which, except for domain B, are highly conserved across isoforms and species. Domain A is contains the phosphorylation site for PKA and CaMKI. Domain B, rich in small amino acids, is considered as a linker region connecting domain A to domain C. Domain C is a large region of about 300 amino acids, containing both hydrophobic and highly charged sequences with the potential to assume α-helix and β-sheet conformations. It shows extensive homology amongst the various members of the family, and mediates the core functions of synapsins1.

Alternative names: Synapsin II (SYN2), Synapsin-2

Gene ID: 29179

NCBI Accession: NM_133625

UniProt: Q63537