Synaptophysin antibody (Intravesicular)

Catalog No. ABIN7043791

This Rabbit Polyclonal antibody specifically detects Synaptophysin in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for Synaptophysin antibody (Intravesicular) (ABIN7043791)

Target: Synaptophysin (SYP)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Synaptophysin Antibody

Binding Specificity: AA 178-190, Intravesicular

Purpose: A Rabbit Polyclonal Antibody to Synaptophysin

Specificity: 2nd intravesicular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Rat,mouse,human - 12,13 amino acid residues identical

Characteristics: Anti-Synaptophysin Antibody (ABIN7043791, ABIN7044656 and ABIN7044657) is a highly specific antibody directed against an epitope of the rat protein. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize synaptophysin from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Comment:

Cited Application: IP|IHC

Negative Control: (ABIN7237323)

Blocking Peptide: (ABIN7237323)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: SYP

Background: Major synaptic vesicle protein p38, SYP,Synaptophysin is the major integral membrane protein of small synaptic vesicles1-3. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini4. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin5. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined6.Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons6.Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles.Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits7,8. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal6.

Alternative names: Synaptophysin, Major synaptic vesicle protein p38, SYP

Gene ID: 24804

NCBI Accession: NM_003179

UniProt: P07825

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity