STX3 antibody (Cytosolic)

Catalog No. ABIN7043795

The Rabbit Polyclonal anti-STX3 antibody has been validated for WB, IF and IC. It is suitable to detect STX3 in samples from Rat.

Quick Overview for STX3 antibody (Cytosolic) (ABIN7043795)

Target: STX3 (Syntaxin 3 (STX3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Grade: KO Validated

Product Details anti-STX3 Antibody

Binding Specificity: AA 2-16, Cytosolic

Purpose: A Rabbit Polyclonal Antibody to Syntaxin 3

Specificity: Cytoplasmic, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Syntaxin 3 Antibody is directed against an epitope of rat Syntaxin 3. Anti-Syntaxin 3 Antibody (ABIN7043795 and ABIN7044652) can be used in western blot and immunocytochemistry applications. It has been designed to recognize Syntaxin 3 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: KDRLEQLKAKQLTQD(C), corresponding to amino acid residues 2-16 of rat syntaxin 3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237343)

Blocking Peptide: (ABIN7237343)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for STX3

Target: STX3 (Syntaxin 3 (STX3))

Alternative Name: STX3

Background: STX3,Syntaxin 3 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2. SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 3, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 3 functions as a plasma membrane protein t-SNARE that is involved in epithelial cell polarization. Indeed, Syntaxin 3 is specifically localized in the apical membrane of polarized epithelial cells where it likely has a role in the polarized trafficking of newly synthesized proteins3.

Alternative names: Syntaxin 3, STX3

Gene ID: 81802

NCBI Accession: NM_004177

UniProt: Q08849

Pathways: Synaptic Vesicle Exocytosis