Syntaxin 4 antibody (Intracellular)

Catalog No. ABIN7043796

Product Details anti-Syntaxin 4 Antibody

Target: Syntaxin 4 (STX4)

Binding Specificity: AA 2-23, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaxin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Purpose: A Rabbit Polyclonal Antibody to Syntaxin 4

Specificity: Intracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 17,22 amino acid residues identical

Characteristics: Anti-Syntaxin 4 Antibody is directed against an epitope of rat Syntaxin 4. Anti-Syntaxin 4 Antibody (ABIN7043796, ABIN7044650 and ABIN7044651)) can be used in western blot and immunohistochemical applications. It was designed to recognize Syntaxin 4 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: RDRTHELRQGDNISDDEDEVRV(C), corresponding to residues 2-23 of rat syntaxin 4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: BLP-NR004

Blocking Peptide: BLP-NR004

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Syntaxin 4

Target: Syntaxin 4 (STX4)

Alternative Name: STX4 (STX4 Products)

Background: STX4, Renal carcinoma antigen NY-REN-31, P35-2,Syntaxin 4 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.Syntaxin 4, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.Syntaxin 4 functions as a plasma membrane protein t-SNARE and has been shown to have an important role in pancreatic b-cell insulin secretion and in insulin-stimulated glucose uptake in skeletal muscle and adipose tissue by means of translocation of the glucose transporter GLUT4 from an intracellular compartment to the plasma membrane3,4.

Alternative names: Syntaxin 4, STX4, Renal carcinoma antigen NY-REN-31, P35-2

Gene ID: 81803

NCBI Accession: NM_004604

UniProt: Q08850

Pathways: Synaptic Vesicle Exocytosis