SNTB1 antibody (Intracellular)

Catalog No. ABIN7043798

The Rabbit Polyclonal anti-SNTB1 antibody is suitable to detect SNTB1 in samples from Mouse. It has been validated for WB.

Quick Overview for SNTB1 antibody (Intracellular) (ABIN7043798)

Target: SNTB1 (Syntrophin, beta 1 (Dystrophin-Associated Protein A1, 59kDa, Basic Component 1) (SNTB1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNTB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SNTB1 Antibody

Binding Specificity: AA 99-113, Intracellular

Purpose: A Rabbit Polyclonal Antibody to β1-Syntrophin (SNTB1)

Specificity: Intracellular, PH1 domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-β1-Syntrophin (SNTB1) Antibody (ABIN7043798, ABIN7045166 and ABIN7045167) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize SNTB1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EQVPESISNQKRGVK, corresponding to amino acid residues 99 -113 of mouse beta1-syntrophin

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:500

Comment:

Negative Control: (ABIN7237354)

Blocking Peptide: (ABIN7237354)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SNTB1

Target: SNTB1 (Syntrophin, beta 1 (Dystrophin-Associated Protein A1, 59kDa, Basic Component 1) (SNTB1))

Alternative Name: SNTB1

Background: Syntrophin-2, SNT2B1, BSYN2, 59 kDa dystrophin-associated protein A1 basic component 1, DAPA1B, Tax interaction protein 43, TIP-43,Syntrophins are a family of five membrane associated adaptor proteins with a molecular weight of 58-60 kDa. Adaptor proteins play a crucial role in coordinating signaling events and may act as rescuers in situations where a subversion from normal signaling exists. Syntrophins are characterized by the presence of a PDZ (postsynaptic density protein-95/disc large/zona occludens-1) split PH (pleckstrin homology) domain and a SU (syntrophin unique) domain within their structure.β-1 Syntrophin is a 538 amino acid long protein while β-2 is 540 amino acid in length. Both forms are ubiquitously expressed in mammalian tissues however β-1 is the predominant form. β-1 Syntrophin is the basic form of the protein while α-1 is acidic. Like all forms of syntrophin, β-1 syntrophin may exist as a monomer or dimer within the cell. Almost all isoforms of syntrophin bind to each other.Syntrophin is required for the proper localization expression and function of NaV1.4 and NaV1.5 Na+ channels. Both channels take part of a multi-protein complex in which dystrophin and syntrophin proteins play an important role in determining their expression levels. NaV1.4 and NaV1.5 C-termini consist of the PDZ domain-binding motif formed by the last three residues. The C-terminus of NaV1.5 thus binds with the dystrophin of DGC and this interaction is mediated by and β-syntrophin (and α-syntrophin) proteins1.Syntrophin binds directly to dystrophin at the vicinity of dystrophin's GPC binding site. Another low affinity binding site is presumed to exist at the COOH-terminal region of dystrophin2.

Alternative names: beta1-Syntrophin (SNTB1), Syntrophin-2, SNT2B1, BSYN2, 59 kDa dystrophin-associated protein A1 basic component 1, DAPA1B, Tax interaction protein 43, TIP-43

Gene ID: 20649

NCBI Accession: NM_021021

UniProt: Q99L88