beta Synuclein antibody (Intracellular)

Catalog No. ABIN7043801

The Rabbit Polyclonal anti-beta Synuclein antibody has been validated for WB. It is suitable to detect beta Synuclein in samples from Rat.

Quick Overview for beta Synuclein antibody (Intracellular) (ABIN7043801)

Target: beta Synuclein (SNCB) (Synuclein, beta (SNCB))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Synuclein antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-beta Synuclein Antibody

Binding Specificity: AA 86-99, Intracellular

Purpose: A Rabbit Polyclonal Antibody to β-Synuclein

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Specific for β-synuclein. No cross-reactivity with α-synuclein.

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Beta-Synuclein Antibody (ABIN7043801, ABIN7045182 and ABIN7045183) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize β-synuclein from human, mouse, and rat samples. The antibody is specific for β-synuclein. It will not cross-react with α-synuclein.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KEEFPTDLKPEEVA, corresponding to amino acid residues 86-99 of rat beta-synuclein

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7237362)

Blocking Peptide: (ABIN7237362)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for beta Synuclein

Target: beta Synuclein (SNCB) (Synuclein, beta (SNCB))

Alternative Name: SNCB

Background: β-Synuclein, Synuclein beta, PNP 14, SNCB,β-Synuclein is a presynaptic protein that plays an important role in neuronal plasticity and synaptic vesicle regulation. The synuclein family includes three members: α, β and γ isoforms. The presynaptic proteins are detected in the neocortex, hippocampus, striatum, thalamus, and cerebellum regions1,2. β-Synuclein has been shown to act as a negative regulator of α-synuclein aggregation both in vivo and in vitro1,2. Structure wise, the protein contains a highly conserved N-terminal domain which includes a repetitive amino acid motif, and a more divergent and highly acidic stretch at the C-terminal domain2,3.Studies have shown overexpressed mRNA levels of β-synuclein in Parkinson's disease suggesting that the protein has a valuable role in maintaining homeostasis in the disease pathology. In addition, studies have shown the action of β-synuclein as a neurodegeneration-inducing factor that can cause cell loss when expressed in rat brains1,2.β-Synuclein is also implicated with dementia involving Lewy bodies. To date, two mutations in the protein, V70M and P123H, have been identified in the sporadic and familial disease3.

Alternative names: Beta-Synuclein, beta-Synuclein, Synuclein beta, PNP 14, SNCB

Gene ID: 113893

NCBI Accession: NM_001001502

UniProt: Q63754