TACR1 antibody (Extracellular) (Atto 488)

Catalog No. ABIN7043804

Product Details anti-TACR1 Antibody (Atto 488)

Target: TACR1 (Tachykinin Receptor 1 (TACR1))

Binding Specificity: AA 180-194, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TACR1 antibody is conjugated to Atto 488

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Neurokinin 1 Receptor Conjugated to the Fluorescent Dye ATTO-488

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,15 amino acid residues identical

Characteristics: Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody (ABIN7043803, ABIN7045318 and ABIN7045319)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and live cell imaging applications. It has been designed to recognize NK1 receptor from rat, mouse, and human samples. \nAnti-Neurokinin 1 Receptor (NK1R) (extracellular)-ATTO Fluor-488 Antibody (#ABIN7043804) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CMIEWPEHPNRTYEK, corresponding to amino acid residues 180-194 of rat NK1 receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:60-1:100

Application Dilutions Western blot wb: N/A

Comment:

Cited Application: FC

Negative Control: RIC-001-AG

Blocking Peptide: BLP-TR001

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TACR1

Target: TACR1 (Tachykinin Receptor 1 (TACR1))

Alternative Name: TACR1 (TACR1 Products)

Background: NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR,Substance P (SP), Neurokinin A (NKA) and Neurokinin B (NKB) are all peptides belonging to the Tachykinin protein family. These three peptides, which demonstrate quite a heterogeneity in their distribution, exert their effect via three receptors: Neurokinin 1-3 receptors, members of the G-protein coupled receptor superfamily. However, Neurokinin 1 (NK1) receptor preferentially binds Substance P, Neurokinin 2 (NK2) receptor to NKA, and Neurokinin 3 (NK3) receptor to NKB1.Neurokinin receptors are distinguished by their seven transmembrane domains, an extracellular N-terminus and a cytosolic C-terminal. An unusual property of these receptors is the presence of introns as part of their structural organization1-3. Tachykinin receptors undergo alternative splicing. For example, NK1 receptor is detected with different C-terminal lengths. The longer receptor isoform is found in the brain whereas the truncated form is mostly detected in the periphery1,4.Due to the broad expression profile of tachykinin peptides, their respective receptors are also expressed in a similar fashion. NK1 receptor is widely expressed in neurons, endothelial cells, muscle and immune system cells. NK2 receptor is broadly expressed in the periphery and its expression in the brain is quite restricted. NK3 receptor on the other hand is largely expressed in the central nervous system and is also detected in the uterus, skeletal muscle, lung and liver1.Neurokinin receptors have been found in many pathophysiological indications and have therefore become targets for the development of pharmacological compounds. Such indications include cancer, psychological disorders, migraine and various inflammations, just to name a few5-8.

Alternative names: Neurokinin 1 Receptor (NK1R), NK1 receptor, Tachykinin receptor 1, TACR1, Substance-P receptor, SPR

Gene ID: 24807

NCBI Accession: NM_001058

UniProt: P14600

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration