TAUT antibody (Intracellular)

Catalog No. ABIN7043807

Product Details anti-TAUT Antibody

Target: TAUT (Taurine Transporter (TAUT))

Binding Specificity: AA 581-594, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAUT antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Taurine Transporter (SLC6A6)

Specificity: Intracellular, C-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Taurine Transporter (SLC6A6) Antibody is directed against an epitope of rat TauT. Anti-Taurine Transporter (SLC6A6) Antibody (ABIN7043807, ABIN7044469 and ABIN7044470)) can be used in western blot analysis. It has been designed to recognize TauT from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)TPREPNRWAVEREG, corresponding to amino acid residues 581-594 of rat TauT

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GT006

Blocking Peptide: BLP-GT006

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TAUT

Target: TAUT (Taurine Transporter (TAUT))

Alternative Name: SLC6A6 (TAUT Products)

Background: Sodium- and chloride-dependent taurine transporter, TauT,Taurine (2-aminoethane-sulfonice acid) is one of the most abundant amino acids found in the human body. It is involved in cell regulation and modulates the concentration of intracellular free calcium. It also plays a substantial role in many other functions varying from neurotransmission in the central nervous system to promotion of cell differentiation in the brain to cytoprotection against oxidative stress1.Taurine is not used for protein synthesis, and in addition to its uptake in nutrition, it can be synthesized in pancreatic mammalian cells by cysteine dioxygenase and sulfinoalanine decarboxylase enzymes2.Taurine is found mainly in organs such as the brain, retina and muscle tissue.The uptake of taurine is conducted through the taurine transporter (TauT). The energy for passage through the transporter is derived by Na+ and Cl- concentration similar to the uptake of other amino acids3. TauT also transports GABA, albeit with a lower affinity. The transporter has twelve transmembrane domains and intracellular N- and C-termini.TauT expression is regulated by cAMP which was found to increase its regulation in MDCK cells and also by PKC via means of post-translational modification. PKC activation leads to reduced taurine transport4. PKC is believed to phosphorylate the highly conserved serine-322 position in the transporter.Taurine deficiency is linked to cardiomyopathies in the hearts of cats and to a decrease in cardiac muscle sensitivity to calcium5 and better understanding of its transport may improve research of these diseases.

Alternative names: Taurine Transporter (SLC6A6), Sodium- and chloride-dependent taurine transporter, TauT

Gene ID: 29464

NCBI Accession: NM_003043

UniProt: P31643