TAUT antibody (C-Term, Intracellular)

Catalog No. ABIN7886476

The Rabbit Polyclonal anti-TAUT antibody is suitable to detect TAUT in samples from Rat. It has been validated for WB.

Quick Overview for TAUT antibody (C-Term, Intracellular) (ABIN7886476)

Target: TAUT (Taurine Transporter (TAUT))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAUT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TAUT Antibody

Binding Specificity: AA 581-594, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Taurine Transporter (SLC6A6)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Taurine Transporter (SLC6A6) Antibody is directed against an epitope of rat TauT. Anti-Taurine Transporter (SLC6A6) Antibody (ABIN7043807, ABIN7044469 and ABIN7044470) can be used in western blot analysis. It has been designed to recognize TauT from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)TPREPNRWAVEREG, corresponding to amino acid residues 581-594 of rat TauT

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for TAUT

Target: TAUT (Taurine Transporter (TAUT))

Alternative Name: SLC6A6

Background:

Synonyms: Sodium- and chloride-dependent taurine transporter, TauT

Description: Taurine (2-aminoethane-sulfonice acid) is one of the most abundant amino acids found in the human body. It is involved in cell regulation and modulates the concentration of intracellular free calcium. It also plays a substantial role in many other functions varying from neurotransmission in the central nervous system to promotion of cell differentiation in the brain to cytoprotection against oxidative stress1.Taurine is not used for protein synthesis, and in addition to its uptake in nutrition, it can be synthesized in pancreatic mammalian cells by cysteine dioxygenase and sulfinoalanine decarboxylase enzymes2.Taurine is found mainly in organs such as the brain, retina and muscle tissue.The uptake of taurine is conducted through the taurine transporter (TauT). The energy for passage through the transporter is derived by Na+ and Cl- concentration similar to the uptake of other amino acids3. TauT also transports GABA, albeit with a lower affinity. The transporter has twelve transmembrane domains and intracellular N- and C-termini.TauT expression is regulated by cAMP which was found to increase its regulation in MDCK cells and also by PKC via means of post-translational modification. PKC activation leads to reduced taurine transport4. PKC is believed to phosphorylate the highly conserved serine-322 position in the transporter.Taurine deficiency is linked to cardiomyopathies in the hearts of cats and to a decrease in cardiac muscle sensitivity to calcium5 and better understanding of its transport may improve research of these diseases.

Gene ID: 29464

NCBI Accession: NM_003043

UniProt: P31643