TMEM119 antibody (Extracellular) (FITC)

Catalog No. ABIN7043860

Product Details anti-TMEM119 Antibody (FITC)

Target: TMEM119 (Transmembrane Protein 119 (TMEM119))

Binding Specificity: AA 53-67, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMEM119 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye FITC

Specificity: Extracellular, N-terminus.

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): This antibody will not recognize human TMEM119

Predicted Reactivity: Rat - 12 out of 15 amino acid residues identical This antibody will not recognize human TMEM119

Characteristics: Anti-TMEM119 (extracellular) Antibody (ABIN7043859, ABIN7044740 and ABIN7044741) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples. \nAnti-TMEM119 (extracellular)-FITC Antibody (ABIN7043859, ABIN7044740 and ABIN7044741)-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7237517)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TMEM119

Target: TMEM119 (Transmembrane Protein 119 (TMEM119))

Alternative Name: TMEM119 (TMEM119 Products)

Background: Transmembrane Protein 119, Osteoblast induction factor, OBIF,Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

Alternative names: Transmembrane Protein 119, Osteoblast induction factor, OBIF

Gene ID: 231633

NCBI Accession: NM_181724

UniProt: Q8R138