TREM2 antibody (Extracellular) (PE)

Catalog No. ABIN7043867

Product Details anti-TREM2 Antibody (PE)

Target: TREM2 (Triggering Receptor Expressed On Myeloid Cells 2 (TREM2))

Binding Specificity: AA 42-56, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TREM2 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to TREM2 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE).

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - 14 out of 15 amino acid residues identical, Rat - 12 out of 15 amino acid residues identical

Characteristics: A Rabbit Polyclonal Antibody to TREM2 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE).

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KHWGRRKAW(S)RQLGE, corresponding to amino acid residues 42-56 of mouse TREM2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582046)

Blocking Peptide: (ABIN7237532)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for TREM2

Target: TREM2 (Triggering Receptor Expressed On Myeloid Cells 2 (TREM2))

Alternative Name: TREM2 (TREM2 Products)

Background: Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on monocytes 2.,TREM2 (triggering receptor expressed on myeloid cells-2) is a type I transmembrane microglial surface receptor that is found to be expressed on myeloid cells including monocyte-derived dendritic cells, osteoclasts and bone-marrow derived macrophage. TREM2 protein transduces intracellular signals that regulate microglial functions such as cytokine production, migration, proliferation, phagocytosis, cell survival, synapse elimination, and compaction of amyloid plaques1,2.The TREM2 structure includes an immunoglobulin-like extracellular domain responsible for ligand binding, a transmembrane region and a short cytoplasmatic tail. The protein also includes an ectodomain that binds anionic and zwitterionic lipids, apolipoproteins and lipoprotein particles2,3.Studies show that TREM2 knockout mice display decreased levels of inflammatory cytokines. TREM2 has been shown to control two signaling pathways in the brain: regulation of phagocytosis and suppression of inflammatory reactivity. Nasu-Hakola disease, a rare and fatal disease, and polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy has been found to be linked to homozygous loss-of-function mutations in TREM2 protein1,2. Studies also show that variants of TREM2 are associated with Alzheimer's disease2,3.

Alternative names: Triggering receptor expressed on myeloid cells 2, Triggering receptor expressed on monocytes 2.

Gene ID: 83433

NCBI Accession: NM_018965

UniProt: Q99NH8