TREM2 antibody (Internal Region)

Catalog No. ABIN570923

The Goat Polyclonal anti-TREM2 antibody is suitable to detect TREM2 in samples from Mouse. It has been validated for ELISA and IF.

Quick Overview for TREM2 antibody (Internal Region) (ABIN570923)

Target: TREM2 (Triggering Receptor Expressed On Myeloid Cells 2 (TREM2))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TREM2 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-TREM2 Antibody

Binding Specificity: Internal Region

Purpose: Trem2 (mouse)

Sequence: QVEHSTSRNQ ET

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QVEHSTSRNQET, from the internal region of the protein sequence according to NP_112544.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments showed a band at approx 26 kDa in Mouse Adrenal gland lysates after 1 μg/mL antibody staining (calculated MW of 24.5 kDa according to Mouse NP_112544.1 and 24.9 according to Rat NP_001100354.1). Primary incubation 1 ho

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Plasma Membrane of NIH3T3-L1 (adipocyte like) cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TREM2

Target: TREM2 (Triggering Receptor Expressed On Myeloid Cells 2 (TREM2))

Alternative Name: Trem2

Background: Trem2, triggering receptor expressed on myeloid cells 2 , Trem2a, Trem2b, Trem2c , triggering receptor expressed on myeloid cells 2a, triggering receptor expressed on myeloid cells 2b, triggering receptor expressed on myeloid cells 2c

Gene ID: 83433, 301227

NCBI Accession: NP_112544