VIPR2 antibody (Extracellular, N-Term)

Catalog No. ABIN7887185

The Rabbit Polyclonal anti-VIPR2 antibody (ABIN7887185) specifically detects VIPR2 in WB, IF, FACS, IC and LCI. The antibody is reactive with Human samples.

Quick Overview for VIPR2 antibody (Extracellular, N-Term) (ABIN7887185)

Target: VIPR2 (Vasoactive Intestinal Peptide Receptor 2 (VIPR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VIPR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-VIPR2 Antibody

Binding Specificity: AA 25-37, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to VPAC2 Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,rat - identical

Characteristics: Anti-VPAC2 (VIPR2) (extracellular) Antibody is directed against an extracellular epitope of human VIP and PACAP receptor 2. Anti-VPAC2 (VIPR2) (extracellular) Antibody (ABIN7043877, ABIN7045331 and ABIN7045332) can be used in western blot analysis, immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize VPAC2 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CRFHLEIQEEETK, corresponding to amino acid residues 25-37 of human VPAC2

Isotype: IgG

Application Details

Application Notes:

WB: 1:600

FC: 2.5 mg per reaction

ICC: 1:50

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IFC

Negative Control: (ABIN7237559)

Blocking Peptide: (ABIN7237559)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for VIPR2

Target: VIPR2 (Vasoactive Intestinal Peptide Receptor 2 (VIPR2))

Alternative Name: VIPR2

Background:

Synonyms: VIP and PACAP receptor 2, Vasoactive intestinal polypeptide receptor 2, Helodermin-preferring VIP receptor, Pituitary adenylate cyclase-activating polypeptide type III receptor, PACAP type III receptor, PACAP-R3

Description: Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating peptide (PACAP) are prominent neuropeptides that are structurally related. Two receptors have similarly high affinity for VIP and PACAP and have therefore been termed VIP and PACAP receptor 1 (VPAC1) and VIP and PACAP receptor 2 (VPAC2). VPAC2 (also known as vasoactive intestinal peptide receptor 2) belongs to the Class II family of G-protein coupled receptors1. A distinct feature of class II GPCRs is the presence of large N-terminal extracellular domains which contain 10 highly conserved amino acids including six cysteines, putative N terminal leader sequences and several potential N-glycosylation sites1.VPAC2 receptor is expressed in the uterus, prostate, smooth muscle of the gastrointestinal tract, seminal vesicles and skin, blood vessels and thymus2. VPAC2 is also expressed in various brain regions including the thalamus and the suprachiasmatic nuclei (SCN)3.VPAC2 plays important roles in the control of mammalian circadian rhythms in the SCN. Mice lacking VPAC2 show altered circadian rhythms in locomotor behavior, neuronal firing and clock gene expression4. Recent studies have also shown that duplication of the VPAC2 gene, and the resulting higher than normal VPAC2 signaling in patients confers a significant risk to schizophrenia5.

Gene ID: 7434

NCBI Accession: NM_003382

UniProt: P41587

Pathways: cAMP Metabolic Process