VAMP2 antibody (Cytosolic)

Catalog No. ABIN7043878

This Rabbit Polyclonal antibody specifically detects VAMP2 in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for VAMP2 antibody (Cytosolic) (ABIN7043878)

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-VAMP2 Antibody

Binding Specificity: AA 2-27, Cytosolic

Purpose: A Rabbit Polyclonal Antibody to VAMP-2

Specificity: Cytoplasmic, N-terminus

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,bovine,Macaca mulata - 25,26 amino acid residues identical

Characteristics: Anti-v Antibody is directed against the intracellular N-terminal domain of rat V. Anti-V Antibody (ABIN7043878, ABIN7044653 and ABIN7044654) can be used in western blot and immunohistochemical applications, and has been designed to recognize V from rat, mouse and human samples.

Purification: The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

Immunogen:

Immunogen: GST fusion protein

Immunogen Sequence: GST fusion protein with the sequence SATAATVPPAAPAGEGGPPAPPPNLT, corresponding to amino acid residues 2-27 of rat VAMP2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:1000

Comment:

Negative Control: (ABIN7566008)

Blocking Peptide: (ABIN7566008)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for VAMP2

Target: VAMP2 (Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2))

Alternative Name: VAMP2

Background: Vesicle-associated membrane protein 2, Synaptobrevin 2, Syb2,VAMP-2 (also known as synaptobrevin-2) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionary conserved stretch of 60-70 amino acids that are arranged in heptad repeats1,2.SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.VAMP-2, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located on the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.VAMP-2 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as the vesicle membrane protein v-SNARE, which together with the plama membrane t-SNARE protein Syntaxin 1 and the membrane-associated SNAP-25 (synaptosome-associated protein 25 kDa), forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2.VAMP-2 is the target of the tetanus neurotoxin (TeNT) and of several botulinum neurotoxin (BoNT) types: type B, D, F, and G. The neurotoxins cause specific proteolytic degradation of the VAMP-2 protein, which in turn causes SNARE complex disruption and inhibition of neurotransmitter release3.

Alternative names: VAMP-2, Vesicle-associated membrane protein 2, Synaptobrevin 2, Syb2

Gene ID: 24803

NCBI Accession: NM_001330125

UniProt: P63045

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport