ZACN antibody (Extracellular)

Catalog No. ABIN7043880

Product Details anti-ZACN Antibody

Target: ZACN (Zinc Activated Ligand-Gated Ion Channel (ZACN))

Binding Specificity: AA 157-170, Extracellular

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZACN antibody is un-conjugated

Application: Western Blotting (WB), Immunochromatography (IC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel

Specificity: Extracellular, N-terminus

Predicted Reactivity: Human

Characteristics: Anti-Human ZACN (extracellular) Antibody (ABIN7043880 and ABIN7045343) is a highly specific antibody directed against an epitope of the human Zinc-activated ligand-gated ion channel. The antibody can be used in western blot, immunocytochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting ZAC in living cells. It has been designed to recognize ZAC from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CNFELLHFPRDHSN, corresponding to amino acid residues 157-170 of human ZACN

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for ZACN

Target: ZACN (Zinc Activated Ligand-Gated Ion Channel (ZACN))

Alternative Name: ZACN (ZACN Products)

Background: Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1,Over forty homologous proteins belong to the superfamily of ligand-gated ion channels. These members are divided into subfamilies, for example: acetylcholine, serotonin and glycine receptors.A new member has been added to this superfamily - ZAC (Zinc-activated channel) which is expressed in humans and dogs but not in rodents1. Like all other ligand-gated ion channels, ZAC has four transmembrane domains and extracellular N- and C-termini. As its name states, the channel is activated by high concentrations of zinc.ZAC mRNA was found to be expressed in the placenta, spinal cord, stomach, and fetal brain1. In another study, ZAC was identified using a computer-assisted program and was detected in similar areas (including pancreas and kidney), but also displayed minor discrepancies2.Being that the channel is expressed in human and dog, deciphering its cellular/physiological functions will be challenging.

Alternative names: ZACN, Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1

Gene ID: 353174

NCBI Accession: NM_180990

UniProt: Q401N2

Pathways: Synaptic Membrane