Recombinant Bax (Active Monomer) (AA 12-24), (AA 3-16) antibody

Catalog No. ABIN7072202

The Rabbit Chimeric anti-Bax (Active Monomer) antibody is suitable to detect Bax (Active Monomer) in samples from Human, Mouse and Rat. It has been validated for IHC, IP and WB.

Quick Overview for Recombinant Bax (Active Monomer) (AA 12-24), (AA 3-16) antibody (ABIN7072202)

Target: Bax (Active Monomer)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Clone: 6A7

Product Details

Binding Specificity: AA 3-16, AA 12-24

Purpose: Anti-Bax (active monomer) [6A7], Rabbit IgG, kappa

Specificity: In the presence of nonionic detergent, the antibody avidly binds the monomeric form of Bax, but not Bax complexed with either Bcl-XL or Bcl-2. [6A7 ] Bax antibody only recognizes conformationally changed active Bax.

Cross-Reactivity: Mouse, Rat

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: KLH conjugated to peptides corresponding to amino acids 3-16 of murine Bax (CGSGEQLGSGGPTSS), amino acids 3-16 of human Bax (CGSGEQPRGGGPTSS), and amino acids 12-24 shared by Bax from both species (CGPTSSEQIMKTGA).

Isotype: IgG kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Bax (Active Monomer)

Background: Bcl-2-like protein 4, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

UniProt: Q07812