Recombinant CD8 alpha/lyt-2 antibody
Reactivity: Mouse
Dep, FACS, IHC, IHC (fro), in vivo
Host: Rat
Chimeric
YTS 169-4
unconjugated
Recombinant Antibody
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- Target
- CD8 alpha/lyt-2
- Antibody Type
- Recombinant Antibody
- Reactivity
- Mouse
-
Host
- Rat
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Clonality
- Chimeric
-
Conjugate
- Un-conjugated
- Application
- Depletion (Dep), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), In vivo Studies (in vivo)
- Purpose
- Anti-CD8 alpha/Lyt-2 [YTS 169.4], Rat IgG2b, kappa
- Specificity
- This antibody recognises the murine CD8 cell surface antigen expressed by a subset of T lymphocytes.
- Characteristics
-
Original Species of Ab: Rat
Original Format of Ab: IgG2b
- Purification
- Protein A affinity purified
- Immunogen
- Murine CD8 (Lyt-2).
- Clone
- YTS 169-4
- Isotype
- IgG2b kappa
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- Application Notes
- The antibody has been shown to show depleting activity in vivo. Mice have two alleles for CD8, Lyt2.1, and Lyt2.2, which are restricted to certain mouse strains. Lyt2.1, for example, is expressed in the mouse strains CBA, AKR, C3H, and DBA, whereas Lyt2.2 is expressed in the mouse strains BALB/c and C57BL/6 (B/6). YTS 169.4 was originally raised against CBA mouse thymocytes. However, there are publications in which it has been used in C57BL/6 and BALB/c mice, wherein it was used specifically as a pan-reactive Lyt2 reagent (Tavaré et al., 2014, PMID: 24390540).
- Comment
-
This is a chimeric antibody created to reduce immunogenicity during in vivo applications.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- CD8 alpha/lyt-2
- Background
- Leu2, CD8, CD8A, CD8a molecule, CD8a antigen, Leu2 T-lymphocyte antigen, Lyt-2, Lyt2OKT8, p32T-cell antigen Leu2, T-cell antigen, T cell co-receptor, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, T8 T-cell antigen
- UniProt
- P01731
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