Recombinant CD8 alpha/lyt-2 antibody

Catalog No. ABIN7072217

The Rat Chimeric anti-CD8 alpha/lyt-2 antibody is suitable to detect CD8 alpha/lyt-2 in samples from Mouse. It has been validated for Dep, FACS, IHC, IHC (fro) and in vivo.

Quick Overview for Recombinant CD8 alpha/lyt-2 antibody (ABIN7072217)

Target: CD8 alpha/lyt-2

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Depletion (Dep), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), In vivo Studies (in vivo)

Clone: YTS 169-4

Product Details

Purpose: Anti-CD8 alpha/Lyt-2 [YTS 169.4], Rat IgG2b, kappa

Specificity: This antibody recognises the murine CD8 cell surface antigen expressed by a subset of T lymphocytes.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: Murine CD8 (Lyt-2).

Isotype: IgG2b kappa

Application Details

Application Notes: The antibody has been shown to show depleting activity in vivo. Mice have two alleles for CD8, Lyt2.1, and Lyt2.2, which are restricted to certain mouse strains. Lyt2.1, for example, is expressed in the mouse strains CBA, AKR, C3H, and DBA, whereas Lyt2.2 is expressed in the mouse strains BALB/c and C57BL/6 (B/6). YTS 169.4 was originally raised against CBA mouse thymocytes. However, there are publications in which it has been used in C57BL/6 and BALB/c mice, wherein it was used specifically as a pan-reactive Lyt2 reagent (Tavaré et al., 2014, PMID: 24390540).

Comment:

This is a chimeric antibody created to reduce immunogenicity during in vivo applications.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: CD8 alpha/lyt-2

Background: Leu2, CD8, CD8A, CD8a molecule, CD8a antigen, Leu2 T-lymphocyte antigen, Lyt-2, Lyt2OKT8, p32T-cell antigen Leu2, T-cell antigen, T cell co-receptor, T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, T8 T-cell antigen

UniProt: P01731