Recombinant LeY antibody

Catalog No. ABIN7072332

The Mouse Monoclonal anti-LeY antibody (Clone BR96) (ABIN7072332) specifically detects LeY in ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant LeY antibody (ABIN7072332)

Target: LeY

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Clone: BR96

Product Details

Purpose: Anti-LeY [BR96], Mouse IgG1, kappa

Specificity: The antibody binds Le(Y) with a Ka of 0.1 μM.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG3

Purification: Protein A affinity purified

Immunogen: H3396 cultured line established from a metastasis of a human breast adenocarcinoma.

Isotype: IgG1 kappa

Application Details

Application Notes: The antibody binds specifically to LeY tetrasaccharide, a carbohydrate antigen expressed at the cell surface of selective carcinomas, including those of the colon, breast, ovary and lung. Studies have shown that the monoclonal antibody has toxic effects to antigen-positive tumour cells, performing ADCC at concentrations down to 0.1 μg/mL. The antibody B396 was also shown to perform complement-dependent cytotoxicity at concentrations of 0.1-5.0 μg/mL in certain carcinoma cell lines. The monoclonal antibody also has the ability to be internalised by antigen-positive tumour cells, and can thereby be used to deliver anti-cancer drugs to the specific tumour cells.

Comment:

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: LeY

Background: Lewis Y