Recombinant GPIIb/IIIa antibody

Catalog No. ABIN7072340

The Rabbit Chimeric anti-GPIIb/IIIa antibody has been validated for ELISA, IF and WB. It is suitable to detect GPIIb/IIIa in samples from Human.

Quick Overview for Recombinant GPIIb/IIIa antibody (ABIN7072340)

Target: GPIIb/IIIa (Platelet Glycoprotein IIb-IIIa Complex (GPIIb/IIIa))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This GPIIb/IIIa antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Clone: 10E5

Product Details anti-GPIIb/IIIa Antibody

Purpose: Anti-GPIIb/IIIa [10E5], Rabbit IgG, kappa

Specificity: The antibody binds to GPIIb/IIIa with an affinity (Kd) of 16.2±6 nM. The antibody completely inhibits platelet aggregation at 7.8 μg/mL.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: Human blood platelets.

Isotype: IgG kappa

Application Details

Application Notes: The antibody binds specifically to human GPIIb/IIIa, and integrin complex of membrane glycoproteins expressed on the surface of platelets. The GPIIb/IIIa complex acts as a receptor for fibrinogen and Von Willebran factor, hemostatic glycoproteins that mediate platelet aggregation and blood coagulation. The monoclonal antibody 10E5 inhibits the binding of fibrinogen to its platelet receptor, preventing the platelet aggregation, to be used during coronary artery surgeries such as angioplasty. The antibody is also useful in the study of the disease Glanzmann's thrombasthenia, caused by defects in GPIIb/IIIa, and of immune thrombocytopenic purpura, where autoantibodies are produced against GPIIb/IIIa.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for GPIIb/IIIa

Target: GPIIb/IIIa (Platelet Glycoprotein IIb-IIIa Complex (GPIIb/IIIa))

Alternative Name: GPIIb/IIIa

Background: Integrin alpha-IIb/beta-3, Glycoprotein IIb/IIIa, αIIbβ3

UniProt: P08514