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Recombinant L-Selectin antibody

This anti-L-Selectin antibody is a Rabbit Chimeric antibody detecting L-Selectin in FACS, WB, IP and BP. Suitable for Human, Cow and Chimpanzee.
Catalog No. ABIN7072350

Quick Overview for Recombinant L-Selectin antibody (ABIN7072350)

Target

See all L-Selectin (SELL) Antibodies
L-Selectin (SELL) (Selectin L (SELL))

Antibody Type

Recombinant Antibody

Reactivity

  • 112
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Human, Cow, Chimpanzee

Host

  • 94
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Rabbit

Clonality

  • 151
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Chimeric

Conjugate

  • 83
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  • 1
  • 1
  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This L-Selectin antibody is un-conjugated

Application

  • 142
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Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Blocking Peptide (BP)

Clone

DREG-56
  • Purpose

    Anti-CD62L [DREG-56], Rabbit IgG, kappa

    Specificity

    This antibody is specific for human L-selectin, a homing receptor selectively expressed on lymphocytes, which allows their entry into secondary lymphoid tissues via high endothelial venules.

    Cross-Reactivity

    Chimpanzee, Cow

    Characteristics

    Original Species of Ab: Mouse

    Original Format of Ab: IgG1

    Purification

    Protein A affinity purified

    Immunogen

    This antibody was produced by immunizing mice with the concentrated supernatant generated after centrifugation of human peripheral blood leukocytes.

    Isotype

    IgG kappa
  • Application Notes

    This antibody has been used in WB, IP and binding assays to define a lymphocyte homing receptor for PLN HEVs (Kishimoto, 1990). In vitro, this antibody has been shown to specifically inhibit over 90 % of binding of human lymphocytes to HEVs within frozen sections of peripheral, but not mucosal, lymphoid tissue (Kishimoto, 1990). This antibody has also been used in labelling studies to demonstate that the trophectoderm of implantation-competent human embryos expresses L-selectin (Genbacev, 2003).

    Comment

    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    PBS with 0.02 % Proclin 300.

    Preservative

    ProClin

    Precaution of Use

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target

    L-Selectin (SELL) (Selectin L (SELL))

    Alternative Name

    CD62L

    Background

    L-selectin, LECAM-1, Ly-22, MEL-14, CD62 antigen-like family member L, Leukocyte adhesion molecule 1, LAM-1, Leukocyte-endothelial cell adhesion molecule 1, LECAM1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen

    UniProt

    P14151
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