Recombinant CD27 antibody

Catalog No. ABIN7072375

This Rabbit Chimeric antibody specifically detects CD27 in FACS, IF, IP, Act. It exhibits reactivity toward Human, Mouse, Rat.

Quick Overview for Recombinant CD27 antibody (ABIN7072375)

Target: CD27 (CD27 Molecule (CD27))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD27 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Activation (Act)

Clone: LG-3A10

Product Details anti-CD27 Antibody

Purpose: Anti-CD27 [LG.3A10], Rabbit IgG, kappa

Specificity: This antibody is specific for mouse CD27, which, as in the human system, is largely restricted to cells of the T cell lineage, and is expressed on the great majority of both αβ and γδ T lymphocytes, and thymocytes.

Cross-Reactivity: Human, Rat

Characteristics:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: This antibody was produced by injecting Armenian hamsters intraperitoneally with a mixture of two murine CD27 tag-expressing Armenian hamster fibroblast clones (AR0524 and AR0530) in Freund's adjuvant

Isotype: IgG kappa

Application Details

Application Notes: This antibody is specific for murine CD27, and has been used to assess the distribution of CD27 expression using IF, FC and IP analysis (Gravstein, 1995). Upon cross-linking, this antibody has been shown to induce a 4-fold increase in T cell proliferation in response to suboptimal stimulation with concanavalin A (Gravestein, 1995), indicating that this antibody is able to mimic ligand binding.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background: TNFRS7, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7

UniProt: P41272

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints