Recombinant CD80 (Galiximab Biosimilar) antibody

Catalog No. ABIN7072388

The Rabbit Chimeric anti-CD80 (Galiximab Biosimilar) antibody has been validated for BR, ELISA, FACS, IHC, IP and WB. It is suitable to detect CD80 (Galiximab Biosimilar) in samples from Human.

Quick Overview for Recombinant CD80 (Galiximab Biosimilar) antibody (ABIN7072388)

Target: CD80 (Galiximab Biosimilar)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD80 (Galiximab Biosimilar) antibody is un-conjugated

Application: Blocking Reagent (BR), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Clone: IDEC-114

Product Details anti-CD80 (Galiximab Biosimilar) Antibody

Purpose: Anti-CD80 [IDEC-114 (Galiximab)], Rabbit IgG, lambda

Specificity: Galiximab is a primatized mAb which consists of human constant and primate (cynomolgus macaque) variable regions and binds specifically to CD80. CD80 is a surface glycoprotein and a member of the B7 family of costimulatory molecules. CD80 antigen regulates T cell activation (through interacting with CD28 or CD152) and is expressed transiently in antigen-presenting cells, T cells and normal B cells, and expressed consitutively on various subtypes of B-cell lymphomas.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: Galiximab was prepared by immunizing cynomolgus monkeys with recombinant CD80 antigen. The variable regions of the light and heavy chains were then cloned by being incorporated into a cassette vector (N5LG1) containing human constant region genes and subsequently transfected into the Dg44 CHO cell line (Hariharan K, 2013)

Isotype: IgG lambda

Application Details

Application Notes: Galiximab acts to decrease in cell proliferation, inhibition of the constitutively active NF-κB pathway, increase in apoptosis and ADCC against various B-cell lymphoma cell lines. Galiximb affects CD28 and CD152 interactions with CD80. This antibody can also be used for FC, WB, ELISA, IP and IHC.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD80 (Galiximab Biosimilar)

Target: CD80 (Galiximab Biosimilar)

Target Type: Biosimilar

Background: B7-1, T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, B7

UniProt: P33681