Recombinant Fibrin beta-Chain antibody
Reactivity: Human
ELISA, BI, in vivo
Host: Rabbit
Chimeric
8E5
unconjugated
Recombinant Antibody
-
- Target
- Fibrin beta-Chain
- Antibody Type
- Recombinant Antibody
- Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Chimeric
-
Conjugate
- Un-conjugated
- Application
- ELISA, BioImaging (BI), In vivo Studies (in vivo)
- Purpose
- Anti-Fibrin beta-chain [8E5], Rabbit IgG, kappa
- Specificity
- 8E5 binds to the amino-terminal region of the β-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. The epitope recognised by this clone is only accessible upon conversion of fibrinogen to fibrin, which entails the proteolytic removal of fibrinopeptides A & B from fibrinogen by thrombin. The antibody is there is therefore fibrin specific. Fibrin is a non-globular protein which plays a role in the clotting of blood and circulates as the precursor fibrinogen. Polymerization occurs through the action of the protease thrombin, which results in the active fibrin. Fibrin polymerizes as an insoluble matrix and forms a clot along with platelets over a wound site.
- Characteristics
-
Original Species of Ab: Mouse
Original Format of Ab: IgG1
- Purification
- Protein A affinity purified
- Immunogen
- 8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic beta-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).
- Clone
- 8E5
- Isotype
- IgG kappa
-
-
- Application Notes
- The recombinant scFv version of 8E5 retains the same antigen-binding activity of the parent antibody. 8E5 can bind to fibrin in the monomeric and polymeric form, both in vivo and ex vivo. This antibody can also be used for the immunodetection of fibrin in vivo. Standard ELISA and competitive ELISA using the epitope peptide has been conducted with both the parental antibody and the scFv.
- Comment
-
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Restrictions
- For Research Use only
-
- Concentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
-
- Target
- Fibrin beta-Chain
- Background
- Factor Ia, factor-1a, fibrin, fibrin β chain, fibrin beta-chain, fibrin beta chain, fibrin β chain, Fibrinopeptide B
- UniProt
- P02675
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