Recombinant Fibrin beta-Chain antibody

Catalog No. ABIN7072407

The Rabbit Chimeric anti-Fibrin beta-Chain antibody (Clone 8E5) (ABIN7072407) specifically detects Fibrin beta-Chain in ELISA, BI and in vivo. The antibody is reactive with Human samples.

Quick Overview for Recombinant Fibrin beta-Chain antibody (ABIN7072407)

Target: Fibrin beta-Chain

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: ELISA, BioImaging (BI), In vivo Studies (in vivo)

Clone: 8E5

Product Details

Purpose: Anti-Fibrin beta-chain [8E5], Rabbit IgG, kappa

Specificity: 8E5 binds to the amino-terminal region of the β-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. The epitope recognised by this clone is only accessible upon conversion of fibrinogen to fibrin, which entails the proteolytic removal of fibrinopeptides A & B from fibrinogen by thrombin. The antibody is there is therefore fibrin specific. Fibrin is a non-globular protein which plays a role in the clotting of blood and circulates as the precursor fibrinogen. Polymerization occurs through the action of the protease thrombin, which results in the active fibrin. Fibrin polymerizes as an insoluble matrix and forms a clot along with platelets over a wound site.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: 8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic beta-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).

Isotype: IgG kappa

Application Details

Application Notes: The recombinant scFv version of 8E5 retains the same antigen-binding activity of the parent antibody. 8E5 can bind to fibrin in the monomeric and polymeric form, both in vivo and ex vivo. This antibody can also be used for the immunodetection of fibrin in vivo. Standard ELISA and competitive ELISA using the epitope peptide has been conducted with both the parental antibody and the scFv.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Fibrin beta-Chain

Background: Factor Ia, factor-1a, fibrin, fibrin β chain, fibrin beta-chain, fibrin beta chain, fibrin β chain, Fibrinopeptide B

UniProt: P02675