Recombinant PDCD1LG2 antibody

Catalog No. ABIN7072432

This Rabbit Chimeric antibody specifically detects PDCD1LG2 in WB, FACS, IP and BR. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant PDCD1LG2 antibody (ABIN7072432)

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This PDCD1LG2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Clone: TY25

Product Details anti-PDCD1LG2 Antibody

Purpose: Anti-PD-L2 [TY25], Rabbit IgG, kappa

Specificity: This antibody binds to mouse PD-L2 and competes with PD-1 binding. It does not cross-react with PD-L1.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising SD rats with B7-DC-transfected RAW264.7 cells and selected on B7-DC-transfected NRK.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used to detect murine PD-L2 by flow cytometry on stimulated dendritic cells as well as various mouse tumour cells. It also detects immunoprecipitates mouse PD-L2 and can be used to detect it by non-reducing Western Blot. TY25 inhibits PD-L2 binding to PD-1 transfected cells (Ansari et al. 2003, PMID: 12847137) and thereby blocks PD-L2 : PD-1 signalling.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for PDCD1LG2

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: PD-L2

Background: CD273, B7-DC, PDL2, B7DC, programmed death ligand 2, CD273, Pdcd1lg2

UniProt: Q9WUL5