Recombinant PDCD1LG2 antibody

Catalog No. ABIN7072433

The Rat Monoclonal anti-PDCD1LG2 antibody is suitable to detect PDCD1LG2 in samples from Mouse. It has been validated for WB, FACS, IP and BR.

Quick Overview for Recombinant PDCD1LG2 antibody (ABIN7072433)

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This PDCD1LG2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Clone: TY25

Product Details anti-PDCD1LG2 Antibody

Purpose: Anti-PD-L2 [TY25], Rat IgG2a, kappa

Specificity: This antibody binds to mouse PD-L2 and competes with PD-1 binding. It does not cross-react with PD-L1.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising SD rats with B7-DC-transfected RAW264.7 cells and selected on B7-DC-transfected NRK.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody has been used to detect murine PD-L2 by flow cytometry on stimulated dendritic cells as well as various mouse tumour cells. It also detects immunoprecipitates mouse PD-L2 and can be used to detect it by non-reducing Western Blot. TY25 inhibits PD-L2 binding to PD-1 transfected cells (Ansari et al. 2003, PMID: 12847137) and thereby blocks PD-L2 : PD-1 signalling.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for PDCD1LG2

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: PD-L2

Background: CD273, B7-DC, PDL2, B7DC, programmed death ligand 2, CD273, Pdcd1lg2

UniProt: Q9WUL5