Recombinant CD35 antibody

Catalog No. ABIN7072442

The Mouse Monoclonal anti-CD35 antibody has been validated for FACS, IF, ELISA, IA and in vivo. It is suitable to detect CD35 in samples from Human.

Quick Overview for Recombinant CD35 antibody (ABIN7072442)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunoassay (IA), In vivo Studies (in vivo)

Clone: HB-8592

Product Details anti-CD35 Antibody

Purpose: Anti-CD35 [HB-8592], Mouse IgG1, kappa

Specificity: This antibody is specific for Human CR1 but does not compete with clones 7G9 or E11, it binds to a site distinct from the C3b binding site and can be used to measure the number of receptors despite occupancy by ligand. This antibody cross-reacts with Chimpanzee, Baboon, Rhesus, and Cynomolgus monkey CR1. The scFv version of this antibody reacted with Mouse CR1.

Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Mouse, Rhesus Monkey

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with purified CR1 from human erythrocytes.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody has been used for the analysis of the expression of the CR1 receptor on macrophages isolated from the peritoneal effluents of CAPD patients with peritonitis and human leukemia-derived CD4⁺ T cell lines by flow cytometry (Hart et al., 1992, PMID: 1606734) (Yaskanin and Waxman, 1995, PMID: 7758124). This antibody was used in immune adherence (IA) binding and release assays (Edberg et al., 1992, PMID: 1376254). This antibody was used in in vivo clearance experiments (Taylor et al., 1997, PMID: 9000042). An scFv version of this antibody was generated, its pharmacokinetics were evaluated, and it was shown to bind mouse mixed lymphocytes and bone marrow-derived dendritic cells in an ELISA and to bind CR1-expressing mouse bone marrow-derived dendritic cells via immunofluorescence labeling (Knight et al., 2008, PMID: 18385459).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35

Background: CR1, C3BR, C4BR, HB8592, HB 8592, MoAb 543

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity