Recombinant CD131 antibody

Catalog No. ABIN7072453

This Mouse Monoclonal antibody specifically detects CD131 in IHC, IP, Func, BP and Crys. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD131 antibody (ABIN7072453)

Target: CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD131 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunoprecipitation (IP), Functional Studies (Func), Blocking Peptide (BP), Crystallization (Crys)

Clone: BION-1

Product Details anti-CD131 Antibody

Purpose: Anti-CD131 [BION-1], Mouse IgG1, kappa

Specificity: Binds to domain 4 of the common beta-chain and recognises the same binding site used by IL-5, GM-CSF and IL-3, or binds to an epitope in close proximity to it. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.

Isotype: IgG1 kappa

Application Details

Application Notes: BION-1 has been shown the block eosinophil production, survival, and activation stimulated by IL-5 as well as by GM-CSF and IL-3, thereby influencing inflammation (Sun et al., 1999, PMID: 10477723). Further, BION-1 has been used in immunohistochemistry experiments in order to examine the presence of GM-CSF-receptor protein in human 8-cell embryos and blastocysts (Sjoblom et al., 2002, PMID: 12444058). In addition, a fab fragment derived from BION-1 has been used in the crystallisation of the common beta-chain to analyse the structure of the activation domain of the GM-CSF/IL-3/IL-5 receptor (Rossjohn et al., 2000, PMID: 10753826).

Comment:

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD131

Target: CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))

Alternative Name: CD131

Background: CSF2RB, CD131, IL3RB, IL-3R common beta subunit, IL5RB, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage), SMDP5, Common Beta-Chain, CDw131, cytokine receptor common subunit beta, beta-c, CD131, Beta Common Chain

UniProt: P32927

Pathways: JAK-STAT Signaling